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PMID: 6770338 Published · ppublish English Comparative Study Journal Article

Decreased sensitivity to amiloride of amphibian epithelia treated with aldosterone. Further evidence for an apical hormonal effect.

Pflugers Archiv : European journal of physiology ·Vol. 383 ·No. 2 ·1980-01-00 ·Pages 151-8

Crabbé J

Abstract

The reversible inhibition of transepithelial sodium transport achieved with amiloride (and triamterene) was evaluated in amphibian preparations stimulated with aldosterone so as to provide further information regarding a possible influence of this hormone on the apical border of target cells. When aldosterone secretion was enhanced by withdrawal of sodium from toad (Bufo marinus) habitat, sensitivity of abdominal skin to amiloride decreased; the same occurred in skin and bladder preparations incubated with aldosterone for several hours. Amiloride proved a less efficient blocker of sodium transport by toad skin exposed to vasopressin and to ouabain; both substances are capable or raising cell sodium content. It is therefore proposed that the decrease in sensitivity to amiloride of amphibian epithelial treated with aldosterone results from an increase in target cell sodium, itself due to a hormone-induced increas in sodium conductance at the apical cell border. Glucose, which enhanced markedly the rate of sodium transport in preparations treated with aldosterone for several hours, failed to decrease any further the response to amiloride; this is taken as an argument for an additional (? secondary) influence of aldosterone on the cell's metabolic machinery connected with the operation of the sodium 'pump'.

MeSH Terms
Aldosterone/pharmacology Amiloride/pharmacology Animals Biological Transport, Active/drug effects Bufo marinus Epithelium/drug effects,metabolism Glucose/metabolism In Vitro Techniques Insulin/pharmacology Pyrazines/pharmacology Skin/drug effects,metabolism Sodium/metabolism Triamterene/pharmacology Urinary Bladder/drug effects,metabolism
Chemicals
Insulin Pyrazines Aldosterone Amiloride Sodium Glucose Triamterene
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Crabbé J
References (37)
37 references, click to expand
  1. An upper limit to the number of sodium channels in frog skin epithelium.
    J Physiol. 1973 Feb;228(3):681-92 PMID: 4540802
  2. Effect of insulin upon the sodium pump in frog skeletal muscle.
    J Physiol. 1973 Jul;232(1):23-45 PMID: 4542575
  3. Characteristics of aldosterone stimulated transport in isolated skin of the toad, Bufo bufo (L.).
    J Steroid Biochem. 1972 Feb;3(2):111-20 PMID: 4631249
  4. Stimulation of active sodium transport across the isolated toad bladder after injection of aldosterone to the animal.
    Endocrinology. 1961 Oct;69:673-82 PMID: 13696161
  5. Mode of action of amiloride in toad urinary bladder. An electrophysiological study of the drug action on sodium permeability of the mucosal border.
    J Membr Biol. 1977 Apr 7;32(1-2):115-32 PMID: 870695
  6. Active transport of sodium as the source of electric current in the short-circuited isolated frog skin.
    Acta Physiol Scand. 1951 Aug 25;23(2-3):110-27 PMID: 14868510
  7. [Influence of aldosterone on sodium transport and transepithelial water flux through isolated ventral skin of Rana temporaria].
    Pflugers Arch. 1970;317(3):236-51 PMID: 5462683
  8. Changes in sodium pool and kinetics of sodium transport in frog skin produced by amiloride.
    Br J Pharmacol. 1970 May;39(1):99-109 PMID: 5420148
  9. The effect of amiloride on sodium transport in the normal and moulting frog skin.
    Acta Physiol Scand. 1970 Jun;79(2):238-43 PMID: 5454893
  10. Identification of aldosterone-induced proteins in the toad's urinary bladder.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):4056-60 PMID: 812087
  11. Amiloride and the sodium channel.
    Naunyn Schmiedebergs Arch Pharmacol. 1974;281(3):261-9 PMID: 4275046
  12. Hormonal influences on transepithelial sodium transport: aldosterone vs. insulin.
    J Steroid Biochem. 1972 Feb;3(2):229-35 PMID: 4631251
  13. Amiloride: a potent inhibitor of sodium transport across the toad bladder.
    J Physiol. 1968 Mar;195(2):317-30 PMID: 5647323
  14. The nature of the frog skin potential.
    Acta Physiol Scand. 1958 Jun 2;42(3-4):298-308 PMID: 13544986
  15. Does intracellular sodium modify membrane permeability to sodium ions?
    Nature. 1977 Mar 31;266(5601):468-9 PMID: 300846
  16. Current-voltage curve of sodium channels and concentration dependence of sodium permeability in frog skin.
    J Physiol. 1977 May;267(1):137-66 PMID: 301566
  17. Proceedings: Aldosterone, moulting and the number of sodium channels in frog skin.
    Br J Pharmacol. 1974 May;51(1):128P-129P PMID: 4548367
  18. Na and K movements across the membranes of frog skin epithelia associated with transient current changes.
    Pflugers Arch. 1975 Jul 21;358(2):159-77 PMID: 1081679
  19. Effect of ouabain on sodium transport across hormone-stimulated toad bladder and skin.
    Pflugers Arch. 1974 Mar 25;347(4):275-96 PMID: 4211960
  20. Wash out characteristics of tracer Na from the transport pool of frog skin.
    Pflugers Arch. 1978 May 31;374(3):235-41 PMID: 307730
  21. THE ACTION OF ALDOSTERONE AND RELATED CORTICOSTEROIDS ON SODIUM TRANSPORT ACROSS THE TOAD BLADDER.
    J Clin Invest. 1964 Apr;43:611-20 PMID: 14149914
  22. PHASE SOLUBILITY TECHNIQUE IN STUDYING THE FORMATION OF COMPLEX SALTS OF TRIAMTERENE.
    J Pharm Sci. 1964 Nov;53:1325-8 PMID: 14253586
  23. Binding of aldosterone to cytoplasmic and nuclear receptors of the urinary bladder epithelium of Bufo marinus.
    Am J Physiol. 1978 Sep;235(3):C82-9 PMID: 211852
  24. THE INFLUENCE OF NA CONCENTRATION ON NA TRANSPORT ACROSS FROG SKIN.
    J Gen Physiol. 1964 May;47:879-93 PMID: 14155434
  25. Electron microprobe analysis of frog skin epithelium: evidence for a syncytial sodium transport compartment.
    J Membr Biol. 1978 Mar 20;39(4):313-31 PMID: 641981
  26. Estimation of the lifespan of amiloride binding sites in the membranes of toad bladder epithelial cells.
    J Physiol. 1976 Mar;255(3):605-18 PMID: 817015
  27. Metabolism and rate of secretion of aldosterone in the bullfrog.
    J Clin Invest. 1969 Nov;47(11):2523-9 PMID: 4304654
  28. Aldosterone-induced protein in toad urinary bladder.
    Science. 1974 Oct 18;186(4160):269-72 PMID: 4212918
  29. The mechanism of action of amipramizide.
    Pflugers Arch. 1968;302(1):79-96 PMID: 5693807
  30. Movement of sodium across the mucosal surface of the isolated toad bladder and its modification by vasopressin.
    J Gen Physiol. 1962 Jan;45:529-43 PMID: 13894805
  31. Some characteristics of the response of the ventral skin of the toad, "Bufo marinus", to aldosterone "in vitro".
    Arch Int Physiol Biochim. 1971 Oct;79(4):805-8 PMID: 4110218
  32. [The mode of action of aldosterone].
    J Physiol (Paris). 1965 Sep-Oct;57(5):600-1 PMID: 5847088
  33. Pharmacological effects in animals and normal human subjects of the diuretic amiloride hydrochloride (MK-870).
    Clin Pharmacol Ther. 1968 May-Jun;9(3):318-27 PMID: 5649984
  34. Membrane effects of antidiuretic hormone.
    Am J Med. 1967 May;42(5):745-56 PMID: 5337376
  35. Effect of changes in transepithelial transport on the uptake of sodium across the outer surface of the frog skin.
    J Gen Physiol. 1971 Aug;58(2):131-44 PMID: 5559619
  36. A hypothesis concerning the mode of action of amiloride and of triamterene.
    Arch Int Pharmacodyn Ther. 1968 Jun;173(2):474-7 PMID: 5684273
  37. Effects of vasopressin and aldosterone on amiloride binding in toad bladder epithelial cells.
    Proc R Soc Lond B Biol Sci. 1975 Jun 17;189(1097):543-75 PMID: 237282
Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00581876
Published
1980-01-00
Pages
151-8
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00581876 View journal details
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