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PMID: 6777758 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Specific changes in Q-ribonucleoside containing transfer RNA species during Friend leukemia cell erythroid differentiation.

Nucleic acids research ·Vol. 8 ·No. 15 ·1980-08-11 ·Pages 3481-9

Lin VK, Farkas WR, Agris PF

Abstract

Changes in specific tRNA isoacceptors during Friend leukemia cell (F.L.C.) erythroid differentiation have been found to be concomitant with differences in the extent of the Q-base modification in certain species of tRNA. Transfer RNA was isolated from F.L.C. cultures after 0, 36, 48, 72, and 96 hr of DMSO induced differentiation. Changes in 17 isoacceptors of tRNAasn, tRNAasp, tRNAhis and tRNAtyr were compared by RPC-5 chromatography. Isoacceptors of these tRNA changed in relative amounts, following consistent trends throughout cell differentiation. The amount and distribution of Q-base containing tRNA isoacceptors was assayed by measuring the quanine-tRNA transferase catalyzed incorporation of [3H]-labeled guanine into tRNA species undermodified in Q-base followed by RPC-5 chormatography of the tRNA. The amount of Q-base containing tRNA species decreased in the first 48 hr after the induction, then increased again, indicating the level of Q-modification is correlated to the process of differentiation. Isoacceptors that lacked the Q-base were eluted late from RPC-5.

MeSH Terms
Animals Cell Differentiation Cell Line Chromatography, High Pressure Liquid Erythropoiesis Friend murine leukemia virus Guanine/metabolism Guanosine/analogs & derivatives Leukemia, Experimental/physiopathology Nucleoside Q/metabolism RNA, Transfer/isolation & purification tRNA Methyltransferases/pharmacology
Chemicals
Guanosine Nucleoside Q Guanine RNA, Transfer tRNA Methyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lin V K
Farkas W R
Agris P F
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.15.3481
Published
1980-08-11
Pages
3481-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324165
Subset
IM
Grants
NCI NIH HHS · IR01 CA16327 · United States
External Links
PubMed source DOI: 10.1093/nar/8.15.3481 PMC Full Text View journal details
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