Home LiteratureArticle Details
PMID: 67782 Published · ppublish English Journal Article

Isolation and study of functional mast cells from lung and mesentery of the guinea pig.

Agents and actions ·Vol. 7 ·No. 1 ·1977-03-00 ·Pages 45-56

Pearce FL, Behrendt H, Blum U, Poblete-Freundt G, Pult P, Stang-Voss C, Schmutzler W

Abstract

A method is described for the dissociation of guinea pig lung and mesentery into their component cells. The method comprises incubation of the tissues with the enzyme pronase in Ca++- and Mg++-free saline followed by mechanical dispersion and yields on average 3-8% free mast cells. These cells are morphologically intact and viable. They retain an active sensitization or can be passively sensitized. The levels of cAMP and cGMP in the whole cell suspension respond to catecholamine, histamine or cholinergic stimulation in a way similar to that observed in the corresponding undispersed tissue. Although the separation of guinea pig mast cells from other cells proved to be more difficult than the purification of rat mast cells, an inrichment of greater than 50% has been achieved. The mast cells isolated by this method proliferate in vitro and have been cultured successfully for several months.

MeSH Terms
Anaphylaxis Animals Cells, Cultured Cyclic AMP/metabolism Cyclic GMP/metabolism Female Guinea Pigs/immunology Histamine Release Immunization, Passive Lung/cytology,immunology Male Mast Cells/immunology,physiology,ultrastructure Mesentery/cytology,immunology Microscopy, Electron Organ Specificity Pronase
Chemicals
Cyclic AMP Pronase Cyclic GMP
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Pearce F L
Behrendt H
Blum U
Poblete-Freundt G
Pult P
Stang-Voss C
Schmutzler W
References (21)
21 references, click to expand
  1. The isolation and some properties of guinea pig mesenteric mast cells.
    Exp Cell Res. 1973 Sep;81(1):156-62 PMID: 4127949
  2. Ultrastructure of guinea pig mast cells.
    J Ultrastruct Res. 1970 Aug;32(3):284-92 PMID: 5459071
  3. The production of IgE and IgG1 antibodies in guinea-pigs immunized with antigen and bacterial lipopolysaccharides.
    Immunology. 1973 Aug;25(2):297-305 PMID: 4354826
  4. Isolation of "biologically intact" mast cells.
    Exp Cell Res. 1959 Nov;18:512-20 PMID: 13840563
  5. A rapid and simple procedure for the determination of guanosine 3',5'-monophosphate by use of the protein-binding method.
    Naunyn Schmiedebergs Arch Pharmacol. 1974;284(1):55-61 PMID: 4372560
  6. Potentiated reagin response to egg albumin and conalbumin in Nippostrongylus brasiliensis infected rats.
    Life Sci. 1969 Oct 15;8(20):1073-7 PMID: 5355421
  7. Separation of epithelial cells from suspensions of cells from the hamster parotid gland in an isokinetic density gradient of Ficoll in tissue culture medium.
    Anal Biochem. 1975 Jun;66(2):353-64 PMID: 1137101
  8. Separation of mammalian cells using programmed gradient sedimentation.
    Exp Mol Pathol. 1969 Oct;11(2):139-52 PMID: 5344066
  9. Cellular mechanisms in anaphylaxis.
    Physiol Rev. 1962 Apr;42:226-70 PMID: 14475373
  10. Effect of anaphylaxis and histamine, pyrilamine and burimamide on levels of cyclic AMP and cyclic GMP in guinea-pig lung.
    Res Commun Chem Pathol Pharmacol. 1974 Aug;8(4):635-51 PMID: 4153648
  11. Studies on the role of c-AMP in the regulation of the anaphylactic histamine release from guinea pig lung.
    Agents Actions. 1973 Oct;3(3):192-3 PMID: 4131670
  12. Many facts, but insufficient knowledge: the story of asthma.
    J Pharm Pharmacol. 1976 Apr;28(4 SUPPL):361-8 PMID: 6740
  13. The in vitro differentiation and culture of normal mast cells from the mouse thymus.
    Ann N Y Acad Sci. 1963 Feb 26;103:20-39 PMID: 13948040
  14. Sympathomimetic bronchodilators and animal models for assessing their potential value in asthma.
    J Pharm Pharmacol. 1976 Apr;28(4 SUPPL):369-74 PMID: 6741
  15. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  16. The histamine content of guinea pig mast cells.
    Experientia. 1960 May 15;16:192 PMID: 13802776
  17. The use of porous plastic filters in the preparation of cell suspensions.
    Microsc Acta. 1977 Jan;79(1):43-5 PMID: 320430
  18. A protein binding assay for adenosine 3':5'-cyclic monophosphate.
    Proc Natl Acad Sci U S A. 1970 Sep;67(1):305-12 PMID: 4318781
  19. Guanosine cyclic 3',5'-monophosphate and guanylate cyclase activity in guinea pig lung: effects of acetylcholine and cholinesterase inhibitors.
    Mol Pharmacol. 1974 Jan;10(1):155-61 PMID: 4152547
  20. A simple, sensitive protein-binding assay for guanosine 3':5'-monophosphate.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):736-9 PMID: 4323786
  21. Dissociation of sensory ganglia from the embryonic chick by pronase and other dispersing agents.
    J Embryol Exp Morphol. 1970 Apr;23(2):519-30 PMID: 5449487
Article Info
Journal
Agents and actions
Abbr.
Agents Actions
ISSN
0065-4299
DOI
10.1007/BF01964880
Published
1977-03-00
Pages
45-56
Language
English
Region
Switzerland
NLM ID
0213341
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01964880
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2