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PMID: 6801182 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Lack of mature B cells in nude mice with X-linked immune deficiency.

The Journal of experimental medicine ·Vol. 155 ·No. 3 ·1982-03-01 ·Pages 903-13

Wortis HH, Burkly L, Hughes D, Roschelle S, Waneck G

Abstract

Mice were bred that simultaneously expressed the mutations nude and x-linked immune deficiency (xid). These doubly deficient animals had less than 10% of normal serum immunoglobulin levels. Their spleen cells did not respond to thymus-independent antigens in vitro nor did they respond to lipopolysaccharide. There was a virtual absence of cells with surface mu, kappa, or lambda 1, as detected by fluorescence. Sections of lymphoid organs revealed an absence of primary B cell follicles. Taken together, these results indicate a lack of mature B cells in nude xid mice. The possibility is considered that mature B cells belong to two subpopulations representing two lineages, one controlled by alleles at the xid locus and the other by alleles at the nude locus.

MeSH Terms
Agammaglobulinemia/genetics,immunology Animals B-Lymphocytes/cytology,immunology Cell Differentiation Female Immunoglobulin kappa-Chains/analysis,biosynthesis Immunoglobulin lambda-Chains/analysis,biosynthesis Leukocyte Count Lymph Nodes/pathology Lymphocyte Activation Lymphocytes Male Mice Mice, Inbred CBA Mice, Nude/genetics Mitogens/pharmacology Receptors, Antigen, B-Cell/analysis,biosynthesis
Chemicals
Immunoglobulin kappa-Chains Immunoglobulin lambda-Chains Mitogens Receptors, Antigen, B-Cell
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wortis H H
Burkly L
Hughes D
Roschelle S
Waneck G
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33 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.155.3.903
Published
1982-03-01
Pages
903-13
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2186628
Subset
IM
Grants
NIAID NIH HHS · AI-15803-02 · United States
PHS HHS · P30-23776-01 · United States
External Links
PubMed source DOI: 10.1084/jem.155.3.903 PMC Full Text View journal details
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