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PMID: 6808512 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Evidence for a role of calmodulin in serum stimulation of Na+ influx in human fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 11 ·1982-06-00 ·Pages 3537-41

Owen NE, Villereal ML

Abstract

Sodium influx in serum-deprived human diploid fibroblasts can be stimulated by addition of serum (5-fold) or the divalent cation ionophore A23187 (3-fold). The possible involvement of calmodulin in serum or A23187 stimulation of Na+ influx has been investigated by using six psychoactive agents that are known to bind calmodulin and inhibit calmodulin-sensitive enzymes. Each agent inhibited serum- and A23187-stimulated Na+ influx in a dose-dependent manner. Furthermore, the K1 for inhibition of serum-stimulated Na+ influx correlates directly with the Ca2+-specific calmodulin binding previously determined in a cell-free system [Levin, R. M. & Weiss, B. (1979) J. Pharmacol. Exp, Ther. 208, 454--459]. None of the agents tested had any effect on the serum-insensitive component of net Na+ influx in these cells. These data support the concept that serum and A23187 stimulate Na+ influx in human diploid fibroblasts via an increase of intracellular Ca2+ and a subsequent calmodulin-mediated activation of the amiloride-sensitive transport pathway.

MeSH Terms
Biological Transport/drug effects Blood Calcimycin/antagonists & inhibitors Calcium-Binding Proteins/physiology Calmodulin/antagonists & inhibitors,physiology Cells, Cultured Culture Media Humans Kinetics Sodium/metabolism Trifluoperazine/pharmacology
Chemicals
Calcium-Binding Proteins Calmodulin Culture Media Trifluoperazine Calcimycin Sodium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Owen N E
Villereal M L
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.11.3537
Published
1982-06-00
Pages
3537-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346456
Subset
IM
Grants
PHS HHS · 06118 · United States
PHS HHS · 28359 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.11.3537 PMC Full Text View journal details
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