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PMID: 214787 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Ca2+-dependent protein phosphorylation system in membranes from various tissues, and its activation by "calcium-dependent regulator".

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 11 ·1978-11-00 ·Pages 5432-6

Schulman H, Greengard P

Abstract

Analysis of membranes from a variety of tissues has revealed a widespread distribution of a protein phosphorylation system dependent on the presence of both Ca2+ and "calcium-dependent regulator" (CDR). This protein phosphorylation system has been studied in some detail in nervous tissue. Neuronal membranes contain a protein phosphorylation system that requires Ca2+ and a soluble heat-stable protein [Schulman, H. & Greengard, P. (1978) Nature (London) 271, 478--479]. This protein has been purified to homogeneity from bovine cerebral cortex, with use of an assay based on its ability to stimulate Ca2+-dependent protein phosphorylation in membranes. This protein kinase activator appears to be identical to CDR of cyclic nucleotide phosphodiesterase. Throughout its purification, this single entity was found to activate both Ca2+-dependent protein kinase and cyclic nucleotide phosphodiesterase. The kinase activator purified here and authentic CDR were equally effective in their ability to activate Ca2+-dependent protein kinase.

MeSH Terms
Animals Calcium/pharmacology Calmodulin/isolation & purification,metabolism Carrier Proteins/metabolism Cattle Cerebral Cortex/metabolism Enzyme Activation Kinetics Male Membrane Proteins/metabolism Phosphoric Diester Hydrolases/metabolism Phosphorylation Protein Kinases/metabolism Rats Tissue Distribution
Chemicals
Calmodulin Carrier Proteins Membrane Proteins Protein Kinases Phosphoric Diester Hydrolases Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schulman H
Greengard P
References (22)
22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.11.5432
Published
1978-11-00
Pages
5432-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392978
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.11.5432 PMC Full Text View journal details
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