Characterization of an RNA-directed DNA polymerase found in association with murine intracytoplasmic A-particles.

Journal of virology ·Vol. 22 ·No. 2 ·1977-05-00 ·Pages 273-80

Kohno M, Tanaka H

Abstract

An RNA-directed DNA polymerase was found to be associated with intracytoplasmic A-particles from DBA/2 mouse leukemia cells. The enzyme activity was detected after disrupting the purified particles with 2 M NaCl-20 mM dithiothreitol. The presence of a divalent cation and all four deoxyribonucleoside triphosphates was essential for this enzyme activity. The enzyme had a clear preference for Mg2+ over Mn2+. Cesium sulfate isopycnic gradient centrifugation of the DNA product synthesized in the actinomycin D-containing reaction revealed the presence of DNA-RNA hybrid. Furthermore, the purified DNA product was found to hybridize with RNA isolated from A-particles. These observations strongly indicate that the endogenous A-particle RNA serves as the template for the DNA polymerase.

MeSH Terms

Animals Culture Techniques Cytoplasm/microbiology DNA, Viral/biosynthesis Enzyme Activation Gammaretrovirus/enzymology,metabolism Leukemia, Experimental Magnesium/pharmacology Manganese/pharmacology Mice Mice, Inbred DBA Neoplasm Transplantation Nucleic Acid Hybridization Polynucleotides RNA-Directed DNA Polymerase/metabolism Surface-Active Agents/pharmacology Templates, Genetic

Chemicals

DNA, Viral Polynucleotides Surface-Active Agents Manganese RNA-Directed DNA Polymerase Magnesium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kohno M

Tanaka H

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28 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.22.2.273-280.1977

Published

1977-05-00

Pages

273-80

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC515716

Subset

External Links

PubMed source DOI: 10.1128/JVI.22.2.273-280.1977 PMC Full Text View journal details

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Characterization of an RNA-directed DNA polymerase found in association with murine intracytoplasmic A-particles.

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