Characterization of DNA polymerase and RNA associated with A-type particles from murine myeloma cells.
The RNA-dependent DNA polymerase present in intracisternal A-type particles from mouse myeloma tumor cells has been studied. This polymerase can use either endogenous A particle RNA or an exogenous synthetic polynucleotide [poly (rA)] as a template. The DNA reaction product is small (4S-10S) and over 90% of it hybridizes to A particle RNA, whereas up to 50% of it hybridizes to murine sarcoma-leukemia virus RNAs. The RNA isolated from purified A particles is generally of low molecular weight (5S-15S) but contains small amount of 70S and 35S components. These results suggest that A-type particles may be related to C-type oncornaviruses.
15 references, click to expand
-
Studies on DNA synthesis in murine myeloma. II. Activation of latent RNA-dependent DNA polymerase activity in membrane fractions.Biochem Biophys Res Commun. 1972 Nov 15;49(4):1093-9 PMID: 4641706
-
Immunoglobulin-producing tumors and myeloma proteins of mice.Physiol Rev. 1972 Jul;52(3):631-719 PMID: 4555516
-
Intracellular and virion 35 S RNA species of murine sarcoma and leukemia viruses.Virology. 1974 May;59(1):258-65 PMID: 4826208
-
A crude nuclease preparation suitable for use in DNA reassociation experiments.Biochim Biophys Acta. 1971 Jul 29;240(4):522-31 PMID: 5123563
-
Characterization of C-type particles produced by a tissue culture-adapted murine myeloma.J Virol. 1973 Dec;12(6):1589-97 PMID: 4128386
-
Characterization of an endogenous RNA-dependent DNA polymerase associated with murine intracisternal A particles.J Virol. 1974 Mar;13(3):712-20 PMID: 4132671
-
The low molecular weight RNAs of Rous sarcoma virus. I. The 4 S RNA.Virology. 1970 Sep;42(1):182-95 PMID: 4318979
-
Distribution of intracisternal A-particles in a variety of normal and neoplastic mouse tissues.Int J Cancer. 1971 Jan 15;7(1):167-75 PMID: 4322935
-
Some structural and antigenic properties of intracisternal A particles occurring in mouse tumors (complement fixation-immunodiffusion-neuroblastoma-plasma-cell tumor).Proc Natl Acad Sci U S A. 1972 Jan;69(1):218-22 PMID: 4333040
-
RNA-directed DNA synthesis and RNA tumor viruses.Adv Virus Res. 1972;17:129-86 PMID: 4348509
-
Characterization of RNA from noninfectious virions produced by sarcoma positive-leukemia negative transformed 3T3 cells.Proc Natl Acad Sci U S A. 1973 Oct;70(10):3002-6 PMID: 4355380
-
Letter: Evidence for non-repetitive subunits in the genome of Rous sarcoma virus.J Mol Biol. 1974 Mar 25;84(1):217-21 PMID: 4364588
-
A novel DNA polymerase activity found in association with intracisternal A-type particles.Proc Natl Acad Sci U S A. 1972 Jun;69(6):1531-6 PMID: 4504367
-
Intracisternal A particles in ova and preimplantation stages of the mouse.Nat New Biol. 1973 May 16;243(124):91-3 PMID: 4512964
-
Analysis of high-molecular-weight ribonucleic acid associated with intracisternal A particles.J Virol. 1973 Feb;11(2):287-98 PMID: 4688704
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
