Abstract
We studied nine solid and two liquid media for their suitability to select Aeromonas and Plesiomonas spp. from human stools, using artificially contaminated samples as well as 254 samples from outpatients with and without diarrhea. Media with optimal sensitivity and specificity for Aeromonas spp. were alkaline peptone-water, Trypticase soy broth with ampicillin, inositol-brilliant green-bile salts agar, dextrin-fuchsin-sulfite agar, xylose-sodium desoxycholate-citrate agar, and Pril-xylose-ampicillin agar. For Plesiomonas sp., alkaline peptone-water and inositol-brilliant green-bile salts agar were optimal. Four strains of Aeromonas spp. were detected in patient samples with these media.
MeSH Terms
Aeromonas/isolation & purification
Ampicillin/pharmacology
Culture Media
Feces/microbiology
Humans
Tolonium Chloride/pharmacology
Vibrionaceae/drug effects,growth & development,isolation & purification
Chemicals
Culture Media
Tolonium Chloride
Ampicillin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
von Graevenitz A
Bucher C
References (12)
12 references, click to expand
-
Pril-xylose-ampicillin agar, a new selective medium for the isolation of Aeromonas hydrophila.
J Med Microbiol. 1979 May;12(2):229-31
PMID: 458841
-
Medium for the isolation of Aeromonas hydrophila.
Appl Microbiol. 1973 Oct;26(4):550-3
PMID: 4584594
-
In-vitro susceptibility of Aeromonas app. to antimicrobial agents.
J Antimicrob Chemother. 1982 Apr;9(4):267-74
PMID: 7085531
-
Membrane filter procedure for enumeration of Aeromonas hydrophila in fresh waters.
Appl Environ Microbiol. 1979 Jul;38(1):108-13
PMID: 485147
-
False-negative oxidase reaction as a result of medium acidification.
Antonie Van Leeuwenhoek. 1980;46(4):301-12
PMID: 7447434
-
Salt-starch xylose lysine deoxycholate agar. A single medium for the isolation of sodium and non-sodium dependent enteric gram-negative bacilli.
Med Microbiol Immunol. 1977 Dec 27;163(4):241-9
PMID: 604729
-
The detection of Aeromonas hydrophila in stool specimens.
Health Lab Sci. 1970 Jul;7(3):124-7
PMID: 4915588
-
A survey of Plesiomonas shigelloides from aquatic environments, domestic animals, pets and humans.
J Hyg (Lond). 1980 Apr;84(2):203-11
PMID: 7358962
-
A taxonomic study of the Aeromonas hydrophila-Aeromonas punctata group.
J Gen Microbiol. 1976 May;94(1):11-22
PMID: 932684
-
Aeromonas hydrophila: ecology and toxigenicity of isolates from an estuary.
J Appl Bacteriol. 1981 Apr;50(2):359-77
PMID: 7251516
-
Nonselectivity of Rimler-Shotts Medium for Aeromonas hydrophila in Estuarine Environments.
Appl Environ Microbiol. 1981 Sep;42(3):544-5
PMID: 16345850
-
Studies on Aeromonas and Plesiomonas species isolated from cases of choleraic diarrhoea.
Indian J Med Res. 1972 Apr;60(4):520-4
PMID: 4660283