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PMID: 6865941 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Growth regulation of melanoma cells by interferon and (2'-5')oligoadenylate synthetase.

Molecular and cellular biology ·Vol. 3 ·No. 5 ·1983-05-00 ·Pages 780-6

Creasey AA, Eppstein DA, Marsh YV, Khan Z, Merigan TC

Abstract

We report that endogenous, as well as exogenous, interferon (IFN) regulates the growth of human melanoma cells in culture. When antibodies directed against human fibroblast IFN were incorporated into the media of high-density cells stimulated to proliferate with serum, the cells entered the cell cycle earlier than did the controls. In investigating the biochemical basis for this finding, we have found that there is an inverse relationship between the (2'-5')oligoadenylate synthetase levels and the percentage of cells in S in untreated cultures. Upon IFN treatment, the relationship is obliterated and (2'-5')oligoadenylate synthetase levels increase throughout all phases of the cell cycle. This increase in enzyme levels correlates well with the decreased probability of the IFN-treated cells to cycle. These findings suggest a biological role for IFN as a negative growth factor for cells in culture.

MeSH Terms
2',5'-Oligoadenylate Synthetase/physiology Antibodies/immunology Cell Cycle/drug effects Cell Division/drug effects Cell Line Humans Interferon Type I/immunology,pharmacology Melanoma/enzymology,pathology
Chemicals
Antibodies Interferon Type I 2',5'-Oligoadenylate Synthetase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Creasey A A
Eppstein D A
Marsh Y V
Khan Z
Merigan T C
References (33)
33 references, click to expand
  1. On the varied biologic effects of interferon.
    Cell Immunol. 1977 Dec;34(2):406-15 PMID: 589672
  2. pppA2'p5'A2'p5'A: an inhibitor of protein synthesis synthesized with an enzyme fraction from interferon-treated cells.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):256-60 PMID: 272640
  3. Synthesis of an oligonucleotide inhibitor of protein synthesis in rabbit reticulocyte lysates analogous to that formed in extracts from interferon-treated cells.
    Eur J Biochem. 1978 Mar;84(1):149-59 PMID: 206430
  4. Inhibition of cell-free protein synthesis by pppA2'p5'A2'p5'A: a novel oligonucleotide synthesized by interferon-treated L cell extracts.
    Cell. 1978 Mar;13(3):565-72 PMID: 657268
  5. Interferon action may be mediated by activation of a nuclease by pppA2'p5'A2'p5'A.
    Nature. 1978 Jun 22;273(5664):684-7 PMID: 208001
  6. Interferon, double-stranded RNA and RNA degradation. Fractionation of the endonucleaseINT system into two macromolecular components; role of a small molecule in nuclease activation.
    Biochem Biophys Res Commun. 1978 Apr 14;81(3):947-54 PMID: 566549
  7. Mechanism of interferon action. Properties of an interferon-mediated ribonucleolytic activity from mouse L929 cells.
    Virology. 1978 Aug;89(1):240-51 PMID: 28606
  8. Interferon action: two distinct pathways for inhibition of protein synthesis by double-stranded RNA.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):5893-7 PMID: 282611
  9. 2-5A synthetase: assay, distribution and variation with growth or hormone status.
    Nature. 1979 Mar 29;278(5703):471-3 PMID: 450053
  10. Accumulation of an mRNA and protein in interferon-treated Ehrlich ascites tumour cells.
    Nature. 1979 Jun 7;279(5713):523-5 PMID: 571963
  11. Fibroblast interferon induces synthesis of four proteins in human fibroblast cells.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1824-7 PMID: 287023
  12. Interferon-induced enzymatic activities and their role in the antriviral state.
    Cell. 1979 Jun;17(2):255-64 PMID: 455463
  13. Activation of a nuclease by pppA2'p5'A2'p5'A in intact cells.
    FEBS Lett. 1979 Sep 1;105(1):47-52 PMID: 226408
  14. Kinetics of the induction of three translation-regulatory enzymes by interferon.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3208-12 PMID: 226962
  15. Increased nuclease activity in cells treated with pppA2'p5'A2'p5' A.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3261-5 PMID: 114998
  16. Interferon action: induction of specific proteins in mouse and human cells by homologous interferons.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4817-21 PMID: 291901
  17. Regulation of lymphocyte mitogenesis by (2'--5') oligo-isoadenylate.
    Nature. 1979 Dec 20-27;282(5741):849-51 PMID: 514363
  18. Interferon effects on Friend leukaemia cells. I. EXpression of virus and erythroid markers in untreated and dimethyl sulphoxide-treated cells.
    J Gen Virol. 1980 Jan;46(1):227-36 PMID: 7351533
  19. Interferon-induced proteins in human fibroblasts and development of the antiviral state.
    J Virol. 1980 May;34(2):446-54 PMID: 6154807
  20. Role of G0-G1 arrest in the inhibition of tumor cell growth by interferon.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1471-5 PMID: 6154934
  21. Virus-specific effects of interferon in embryonal carcinoma cells.
    Nature. 1980 Jul 10;286(5769):178-80 PMID: 6157099
  22. Natural occurrence of 2-5A in interferon-treated EMC virus-infected L cells.
    Nature. 1979 Dec 6;282(5739):582-6 PMID: 95208
  23. Abnormal behavior of interferon-induced enzymatic activities in an interferon-resistant cell line.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4479-83 PMID: 6159629
  24. Increased levels of (2'-5')oligo(A) polymerase activity in human lymphoblastoid cells treated with glucocorticoids.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6506-10 PMID: 6161367
  25. Mechanism of interferon action. Interferon-mediated inhibition of reovirus mRNA translation in the absence of detectable mRNA degradation but in the presence of protein phosphorylation.
    Virology. 1980 Dec;107(2):461-475 PMID: 6161476
  26. Anti-mitogenic function of interferon-induced (2'-5')oligo(adenylate) and growth-related variations in enzymes that synthesize and degrade this oligonucleotide.
    Eur J Biochem. 1981;114(1):5-10 PMID: 6163628
  27. Ribosomal RNA cleavage, nuclease activation and 2-5A(ppp(A2'p)nA) in interferon-treated cells.
    Nucleic Acids Res. 1981 Apr 10;9(7):1571-81 PMID: 6164990
  28. Enzymatic activities induced by interferon in human fibroblast cell lines differing in their sensitivity to the anticellular activity of interferon.
    Virology. 1981 May;111(1):11-22 PMID: 6165134
  29. Role of interferon-induced enzymes in the antiviral and antimitogenic effects of interferon.
    Ann N Y Acad Sci. 1980;350:459-72 PMID: 6165297
  30. Regulation of 2'5'-oligo(A) polymerase activity in quiescent human fibroblasts treated with interferon.
    Virology. 1981 Jun;111(2):666-70 PMID: 6166120
  31. The importance of G0 in the site of action of interferon in the cell cycle.
    Exp Cell Res. 1981 Jul;134(1):155-60 PMID: 6166490
  32. Antiproliferative effect of (2'-5') oligoadenylate distinct from that of interferon in lymphoid cells.
    Biochem Biophys Res Commun. 1982 Jul 30;107(2):511-7 PMID: 6181787
  33. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.3.5.780-786.1983
Published
1983-05-00
Pages
780-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368600
Subset
IM
Grants
NIAID NIH HHS · AI-05629 · United States
External Links
PubMed source DOI: 10.1128/mcb.3.5.780-786.1983 PMC Full Text View journal details
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