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PMID: 6894331 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The chromosomal arrangement of two linked actin genes in the sea urchin S. purpuratus.

Nucleic acids research ·Vol. 9 ·No. 3 ·1981-02-11 ·Pages 591-604

Schuler MA, Keller EB

Abstract

Four distinct actin genes of the sea urchin Strongylocentrotus purpuratus have been isolated from a recombinant Charon 4 phage library of genomic DNA. The four genes differ considerably from each other in many of their restriction sites. Two of the four genes are closely linked; they are present in the same fragment of cloned DNA. This fragment has been extensively mapped, and some parts of the DNA have been sequenced. The two linked genes are oriented in the same direction, separated by 7.5 kb of DNA. One has an intron following the CAG that codes for the glutamine residue at position 121 in the amino acid sequence of actin. This represents the fifth distinct site at which introns have been found in actin genes, suggesting that the primordial actin gene had at least 6 exons and 5 introns. The actin genes from a distinctive family in which most introns have apparently been precisely excised from the genes.

MeSH Terms
Actins/genetics Animals Bacteriophages/genetics Base Sequence Chromosome Mapping DNA/analysis DNA, Recombinant DNA, Viral Genes Nucleic Acid Hybridization Plasmids Sea Urchins/genetics Thermodynamics
Chemicals
Actins DNA, Recombinant DNA, Viral DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schuler M A
Keller E B
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29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.3.591
Published
1981-02-11
Pages
591-604
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327224
Subset
IM
Grants
NIGMS NIH HHS · GM 10791 · United States
Databases
GENBANK
J01168, J01200
External Links
PubMed source DOI: 10.1093/nar/9.3.591 PMC Full Text View journal details
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