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PMID: 68990 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

B-lymphocytes activation by the Fc region of IgG.

The Journal of experimental medicine ·Vol. 146 ·No. 1 ·1977-07-01 ·Pages 241-56

Berman MA, Weigle WO

Abstract

Strong stimulation of DNA synthesis (up to 150-fold) and blast transformation can be induced in mouse spleen cells by Fc fragments of human IgG. The mitogenic response is optimal on day 5 of culture and is dependent on the concentration of Fc fragments with a sedimentation rate of 3-5S. Intact IgG is also stimulatory, but only when modified by heat aggregation, and produces only a 10-fold increase in [3H]thymidine uptake. The stimulation by aggregated IgG is dependent on the Fc portion, since aggregated (or soluble) Fab or F(ab')2 fragments are inactive. The results show that the response is T-cell independent and that it is a function of nylon wool adherent, surface Ig-positive, Fc receptor-bearing B lymphocytes. Fc fragments do not induce plaque-forming cells to human IgG in normal mouse spleen cell cultures, but rather trigger polyclonal antibody synthesis (anti-goat erythrocytes, anti-2,4,6-trinitrophenyl). It is postulated that the Fc region of antibodies plays a role in the regulation of the humoral immune response by triggering clonal expansion of B lymphocytes.

MeSH Terms
Animals Antibody Formation Antilymphocyte Serum/metabolism B-Lymphocytes/immunology Cell Division/drug effects Complement System Proteins Concanavalin A/pharmacology Erythrocytes/immunology Immunoglobulin Fc Fragments Immunoglobulin G Lipopolysaccharides/pharmacology Lymphocyte Activation/drug effects Male Mice Mice, Inbred A Nylons Spleen/cytology T-Lymphocytes/immunology Thymidine/metabolism Trinitrobenzenes/immunology gamma-Globulins
Chemicals
Antilymphocyte Serum Immunoglobulin Fc Fragments Immunoglobulin G Lipopolysaccharides Nylons Trinitrobenzenes gamma-Globulins Concanavalin A Complement System Proteins Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Berman M A
Weigle W O
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42 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.146.1.241
Published
1977-07-01
Pages
241-56
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2180743
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.146.1.241 PMC Full Text View journal details
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