Home LiteratureArticle Details
PMID: 691108 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcription of adenovirus RNA in permissive and nonpermissive infections.

Journal of virology ·Vol. 27 ·No. 1 ·1978-07-00 ·Pages 136-48

Farber MS, Baum SG

Abstract

The mechanism of blocked replication of human adenoviruses in monkey cells was examined. Previous experiments have placed the replicative block at the level of transcription of translation of adenovirus mRNA. Coinfection of the monkey cells with simian virus 40 enhances adenovirus replication in these cells. We compared the adenovirus mRNA transcribed during infection of permissive human cells and enhanced and unenhanced monkey cells. Adenovirus mRNA from enhanced monkey cells appeared to be identical to adenovirus mRNA from human cells. This indicated that simian virus 40 coinfection did not overcome the blocked replication by substituting for a missing adenovirus transcript. Comparison of adenovirus mRNA from enhanced and unenhanced monkey cell infection revealed two types of transcriptional discrepancies. There was a decrease in both the complexity and the relative abundance of several regions of the enhanced adenovirus mRNA. However, neigher of these transcriptional defects was sufficient to totally explain the difference in yield of infectious virus and viral protein seen in these two types of infection.

MeSH Terms
Adenoviruses, Human/genetics,growth & development,metabolism Cell Line Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/genetics RNA, Viral/genetics Transcription, Genetic Viral Proteins/biosynthesis
Chemicals
RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Farber M S
Baum S G
References (36)
36 references, click to expand
  1. Adenovirus strand nomenclature: a proposal.
    J Virol. 1977 Jun;22(3):830-1 PMID: 875138
  2. The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: mapping of nascent RNA molecules labeled in isolated nuclei.
    Cell. 1977 Apr;10(4):611-6 PMID: 862022
  3. Messenger RNA for myosin polypeptides: isolation from single myogenic cell cultures.
    Cell. 1977 Feb;10(2):265-73 PMID: 837449
  4. Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments.
    Proc Natl Acad Sci U S A. 1975 Apr;72(4):1344-8 PMID: 1093178
  5. Quantitative deficiency of chain-specific globin messenger ribonucleic acids in the thalassemia syndromes.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1809-13 PMID: 4124305
  6. Specificity in initiation of protein synthesis in a fractionated mammalian cell-free system.
    Nat New Biol. 1973 Apr 4;242(118):136-40 PMID: 4349564
  7. Posttranscriptional block to adenovirus replication in nonpermissive monkey cells.
    Virology. 1974 Jul;60(1):45-53 PMID: 4366494
  8. An SV40-induced initiation factor for protein synthesis concerned with the regulation of permissiveness.
    Nature. 1974 Dec 20;252(5485):649-53 PMID: 4373655
  9. Complementation of translational defect for growth of human adenovirus type 2 in Simian cells by a Simian virus 40-induced factor.
    J Mol Biol. 1973 Dec 5;81(2):207-23 PMID: 4360026
  10. Studies of the mechanism of enhancement of human adenovirus infection in monkey cells by simian virus 40.
    J Virol. 1972 Aug;10(2):211-9 PMID: 4342239
  11. Specificity of the break produced by restricting endonuclease R 1 in Simian virus 40 DNA, as revealed by partial denaturation mapping.
    Proc Natl Acad Sci U S A. 1972 Nov;69(11):3215-9 PMID: 4343959
  12. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
    Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
  13. Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: formation of adenovirus-specific RNA.
    Virology. 1968 Feb;34(2):373-6 PMID: 4295919
  14. Interaction of a simian papovavirus and adenoviruses. I. Induction of adenovirus tumor antigen during abortive infection of simian cells.
    J Bacteriol. 1966 Feb;91(2):813-8 PMID: 4286827
  15. Nucleic acid homology studies of adenovirus type 7-SV40 interactions.
    Proc Natl Acad Sci U S A. 1966 Feb;55(2):336-41 PMID: 4287386
  16. Synthesis of virus-specific ribonucleic acid in KB cells infected with type 2 adenovirus.
    J Virol. 1971 Aug;8(2):203-14 PMID: 4256015
  17. Synthesis of complementary RNA sequences during productive adenovirus infection.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4887-91 PMID: 4612530
  18. The alteration of ribosomes for mRNA selection concerned with adenovirus growth in SV40-infected simian cells.
    Virology. 1975 Sep;67(1):85-93 PMID: 170741
  19. Block to multiplication of adenovirus serotype 2 in monkey cells.
    J Virol. 1975 Dec;16(6):1650-68 PMID: 172661
  20. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  21. Translation of myoglobin messenger ribonucleic acid.
    Biochemistry. 1973 Nov 6;12(23):4559-65 PMID: 4797988
  22. Messenger RNA in HeLa cells: kinetics of formation and decay.
    J Mol Biol. 1973 Aug 5;78(2):321-34 PMID: 4747634
  23. Processing of adenovirus 2-induced proteins.
    J Virol. 1973 Aug;12(2):241-52 PMID: 4747985
  24. Synthesis of vaccinia virus "early" and "late" messenger RNA in vitro with nucleoprotein structures isolated from infected cells.
    Virology. 1970 Oct;42(2):463-72 PMID: 4923015
  25. T7 protein synthesis in F-factor-containing cells: evidence for an episomally induced impairment of translation and relation to an alteration in membrane permeability.
    J Virol. 1975 Jan;17(1):94-105 PMID: 1107598
  26. Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:457-74 PMID: 1057474
  27. Bidirectional replication of adenovirus type 2 DNA.
    J Virol. 1976 Apr;18(1):307-15 PMID: 1255873
  28. Studies concerning the mechanism by which translational-control RNA regulates protein synthesis in embryonic muscle.
    Eur J Biochem. 1975 Oct 15;58(2):587-93 PMID: 1237405
  29. Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H.
    J Mol Biol. 1975 Sep 5;97(1):123-5 PMID: 1177312
  30. A crude nuclease preparation suitable for use in DNA reassociation experiments.
    Biochim Biophys Acta. 1971 Jul 29;240(4):522-31 PMID: 5123563
  31. Intermediates in the synthesis of type 2 adenovirus deoxyribonucleic acid.
    J Virol. 1971 Nov;8(5):675-83 PMID: 5132696
  32. Isolation and partial characterization of reticulocyte factors M1 and M2.
    J Biol Chem. 1970 Nov 10;245(21):5553-9 PMID: 5472357
  33. Fractions of HeLa DNA differing in their content of guanine+cytosine.
    J Mol Biol. 1969 Dec 14;46(2):305-12 PMID: 5360041
  34. Persistence of messenger RNA through mitosis in HeLa cells.
    J Cell Biol. 1969 Feb;40(2):497-507 PMID: 5761922
  35. The ribonuclease activity of crystallized pancreatic deoxyribonuclease.
    Anal Biochem. 1966 Feb;14(2):269-77 PMID: 5939865
  36. Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ.
    Nucleic Acids Res. 1974 Nov;1(11):1385-97 PMID: 10793698
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.27.1.136-148.1978
Published
1978-07-00
Pages
136-48
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354147
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.27.1.136-148.1978 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2