The nucleotide sequences of the initiator transfer RNAs from bean cytoplasm and chloroplasts.

Nucleic acids research ·Vol. 8 ·No. 5 ·1980-03-11 ·Pages 999-1008

Canaday J, Guillemaut P, Weil JH

Abstract

The initiator tRNAsMet from the cytoplasm and chloroplasts of Phaseolus vulgaris have been purified and sequenced. The sequence of bean cytoplasmic initiator tRNAiMet is : pA-U-C-A-G-A-G-U-m1G-m2G-C-G-C-A-G-C-G-G-A-A-G-C-G-U-m2G-G-U-G-G-G2-C-C-C-A-U-t6A-A-C-C-C-A-C-A-G-m7G-D-m5C-C-C-A-G-G-A-psi-C-G-m1A-A-A-C-C-U-Gm-G-C-U-C-U-G-A-U-A-C-C-AOH. The sequence of bean cytoplasmic tRNAiMet is almost identical to that of wheat germ and shows a high degree of homology with other cytoplasmic initiator tRNAs. The sequence of bean chloroplast initiator tRNAfMet is : pC-G-C-G-G-A-G-U-A-G-A-G-C-A-A-C-U-U-Gm-G-D-A-G-C-U-C-G-C-A-A-G-G-C-U-C-A-U-A-A-C-C-U-U-G-A-A-m7G-acp3U-U-A-C-G-G-G-T-psi-C-A-A-A-U-C-C-C-G-U-C-U-C-C-G-C-A-A- C-C-AOH. Bean chloroplast initiator tRNAfMet sequence shows procaryotic characteristics at the 5' end of the acceptor stem and in the TpsiC loop, but also contains some distinctive features.

MeSH Terms

Base Sequence Chloroplasts/metabolism Cytoplasm/metabolism Eukaryotic Cells/metabolism Fabaceae/genetics Nucleic Acid Conformation Plants, Medicinal Prokaryotic Cells/metabolism RNA, Transfer, Amino Acyl/genetics RNA, Transfer, Met Species Specificity

Chemicals

RNA, Transfer, Amino Acyl RNA, Transfer, Met tRNA(m)(Met), methionine- tRNA, formylmethionine-

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Canaday J

Guillemaut P

Weil J H

References (27)

27 references, click to expand

The use of nuclease P1 in sequence analysis of end group labeled RNA.
Nucleic Acids Res. 1977 Dec;4(12):4091-108 PMID: 202925
Studies on human tRNA. I. The rapid, large scale isolation and partial fractionation of placenta and liver tRNA.
Nucleic Acids Res. 1975 Jan;2(1):21-42 PMID: 236551
Nucleotide sequence of starfish initiator tRNA.
Nucleic Acids Res. 1979 Aug 10;6(11):3459-69 PMID: 386274
Studies on the sequence of the 3'-terminal region of turnip-yellow-mosaic-virus RNA.
Eur J Biochem. 1977 Feb;72(3):465-78 PMID: 402264
Presence of N-formyl-methionyl-transfer RNA in bean chloroplasts.
FEBS Lett. 1969 Aug;4(4):285-287 PMID: 11947205
The primary structure of cytoplasmic initiator transfer ribonucleic acid from Torulopsis utilis.
J Biochem. 1979 Aug;86(2):335-46 PMID: 573264
Formylation of mischarged E. coli tRNA Met f .
FEBS Lett. 1973 Mar 15;30(3):291-5 PMID: 4573437
The first nucleotide sequence of an organelle transfer RNA: chloroplastic tRNAphe.
Cell. 1976 Dec;9(4 PT 2):717-23 PMID: 828079
Sequence analysis of 5'[32P] labeled mRNA and tRNA using polyacrylamide gel electrophoresis.
Nucleic Acids Res. 1978 Jan;5(1):37-56 PMID: 25417
Evidence against the folate-mediated formylation of formyl-accepting methionyl transfer ribonucleic acid in Streptococcus faecalis R.
J Biol Chem. 1970 Oct 10;245(19):5115-21 PMID: 4990168
Nucleotide sequence of Neurospora crassa cytoplasmic initiator tRNA.
Nucleic Acids Res. 1977 Dec;4(12):4109-31 PMID: 146192
Mapping adenines, guanines, and pyrimidines in RNA.
Nucleic Acids Res. 1977 Aug;4(8):2527-38 PMID: 409999
Separation of transfer ribonucleic acid by sepharose chromatography using reverse salt gradients.
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1068-71 PMID: 1093164
Conservation of the structures of plant tRNAs and aminoacyl-tRNA synthetases.
FEBS Lett. 1979 Jun 1;102(1):194-7 PMID: 456588
The use of thin acrylamide gels for DNA sequencing.
FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
Preparative two-dimensional polyacrylamide gel electrophoresis of 32 P-labeled RNA.
Anal Biochem. 1972 Sep;49(1):184-97 PMID: 5081097
Compilation of tRNA sequences.
Nucleic Acids Res. 1979 Jan;6(1):r1-r19 PMID: 424282
Effect of formylation on the chromatographic behavior of methionyl transfer ribonucleic acid.
Anal Biochem. 1972 May;47(1):244-52 PMID: 4624155
3-(3-Amino-3-carboxypropyl)uridine: a novel modified nucleoside isolated from Escherichia coli phenylalanine transfer ribonucleic acid.
Biochemistry. 1974 Jun 4;13(12):2620-5 PMID: 4598734
Nucleotide sequence of salmon testes and salmon liver cytoplasmic initiator tRNA.
Cell. 1975 Nov;6(3):395-405 PMID: 1053250
Primary structure of bean chloroplastic tRNAPhe. Comparison with Euglena chloroplastic tRNAPhe.
FEBS Lett. 1977 Dec 15;84(2):351-6 PMID: 413742
Aminoacylation of Phaseolus vulgaris cytoplasmic, chloroplastic and mitochondrial tRNAsMet and of Escherichia coli tRNAsMet by homologous and heterologous enzymes.
Biochim Biophys Acta. 1975 Oct 1;407(2):240-8 PMID: 1101967
A different approach to RNA sequencing.
Nature. 1978 Jul 6;274(5666):87-9 PMID: 662002
Initiator tRNAs have a unique anticodon loop conformation.
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3289-93 PMID: 386336
The nucleotide sequence of the initiator tRNA from Drosophila melanogaster.
Nucleic Acids Res. 1979 Feb;6(2):421-33 PMID: 106370
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.
Nucleic Acids Res. 1974 Mar;1(3):331-53 PMID: 10793670
Mapping of yeast tRNAs by two-dimensional electrophoresis on polyacrylamide gels.
FEBS Lett. 1975 Feb 1;50(2):185-9 PMID: 1089563

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/8.5.999

Published

1980-03-11

Pages

999-1008

Language

English

Region

England

NLM ID

0411011

PMCID

PMC323968

Subset

Databases

GENBANK

K00318, K00319

External Links

PubMed source DOI: 10.1093/nar/8.5.999 PMC Full Text View journal details

Analysis Services

The nucleotide sequences of the initiator transfer RNAs from bean cytoplasm and chloroplasts.

Contact

WeChat Official Account