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PMID: 6929401 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetic analysis of bacteriophage P4 using P4-plasmid ColE1 hybrids.

Molecular & general genetics : MGG ·Vol. 177 ·No. 3 ·1980-02-00 ·Pages 399-412

Kahn M, Ow D, Sauer B, Rabinowitz A, Calendar R

Abstract

A set of plasmids that contain fragments of the bacteriophage P4 genome has been constructed by deleting portions of a P4-ColE1 hybrid. A P4 genetic map has been established and related to the physical map by examining the ability of these plasmids to rescue various P4 mutations. The P4 virl mutation and P4 genes involved in DNA replication (alpha), activation of P2 helper genes (delta and epsilon), polarity suppression (psu) and head size determination (sid) have been mapped, as has the region responsible for synthesis of a nonessential P4 protein. One of the deleted plasmids contains only 5900 base pairs (52%) of P4 but will form plaques if additional DNA is added to increase its total size to near that of P4. This plasmid is also unique in that it will not form stable associations with P2 lysogens of E. coli which are recA+. P4 alpha mutants can be suppressed as a result of replication under control of the ColE1 part of the hybrid.

MeSH Terms
Chromosome Mapping Coliphages/genetics,ultrastructure DNA Replication DNA, Bacterial DNA, Viral Genes Nucleic Acid Hybridization Plasmids Recombination, Genetic Viral Proteins/biosynthesis,genetics
Chemicals
DNA, Bacterial DNA, Viral Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kahn M
Ow D
Sauer B
Rabinowitz A
Calendar R
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47 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00271478
Published
1980-02-00
Pages
399-412
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00271478
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