Limited DNase I nicking as a probe of gene conformation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 4 ·1980-04-00 ·Pages 1907-11

Zasloff M, Camerini-Otero RD

Abstract

We have extended the use of pancreatic DNase I as a probe of chromatin structure by exploring the accessibility of an active gene to the introduction of the first single-stranded nick. We show by a target analysis that the beta-globin gene is about 25-fold more sensitive to single-site nicking than is an average sequence in the chicken erythrocyte nucleus or the nontranscribed albumin gene. The sites of initial DNase I nicking are shown to cluster within the transcribed sequence of the beta-globin gene.

MeSH Terms

Albumins/genetics Animals Base Sequence Chickens Chromatin/ultrastructure Deoxyribonucleases/metabolism Erythrocytes/physiology Female Genes Globins/genetics Molecular Weight Nucleosomes/ultrastructure Oviducts/physiology Transcription, Genetic

Chemicals

Albumins Chromatin Nucleosomes Globins Deoxyribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Zasloff M

Camerini-Otero R D

References (27)

27 references, click to expand

Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
Structural studies on chick embryonic hemoglobins.
J Biol Chem. 1974 Jun 25;249(12):3960-72 PMID: 4134752
Nucleotide sequence of the rightward operator of phage lambda.
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Chromosomal subunits in active genes have an altered conformation.
Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3966-70 PMID: 1069279
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
Renaturation kinetics of cDNA complementary to cytoplamic polyadenylated RNA from rainbow trout testis. Accessibility of transcribed genes to pancreatic DNase.
Nucleic Acids Res. 1977 Apr;4(4):883-98 PMID: 866196
Selective degradation of integrated murine leukemia proviral DNA by deoxyribonucleases.
Cell. 1977 Aug;11(4):933-40 PMID: 560916
Analysis of in vitro transcription of duck reticulocyte chromatin using mercury-substituted ribonucleoside triphosphates.
Biochemistry. 1977 Nov 15;16(23):5135-45 PMID: 334258
A physical map of the DNA regions flanking the rabbit beta-globin gene.
Cell. 1977 Oct;12(2):429-39 PMID: 912752
An altered subunit configuration associated with the actively transcribed DNA of integrated adenovirus genes.
Cell. 1977 Nov;12(3):783-94 PMID: 562719
Genes transcribed at diverse rates have a similar conformation in chromatin.
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4867-71 PMID: 270719
A new method for the purification and identification of covalently closed circular DNA molcules.
Nucleic Acids Res. 1978 Apr;5(4):1139-52 PMID: 26042
Regulation of gene expression in the chick oviduct by steroid hormones.
Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:639-47 PMID: 277311
Active conformation of the globin genes in uninduced and induced mouse erythroleukemia cells.
Cell. 1978 Jul;14(3):511-21 PMID: 99241
Regulation of expression and chromosomal subunit conformation of avian retrovirus genomes.
Cell. 1978 Aug;14(4):865-78 PMID: 210959
Degradation of the ribosomal genes by DNAse I in Physarum polycephalum.
Eur J Biochem. 1978 Oct;90(2):391-5 PMID: 710437
Analysis of the adult and embryonic chicken globin genes in chromosomal DNA.
J Biol Chem. 1978 Nov 25;253(22):8239-46 PMID: 711749
Cloning of a double-stranded cDNA that codes for a portion of chicken preproalbumin. A general method for isolating a specific DNA sequence from partially purified mRNA.
J Biol Chem. 1978 Dec 10;253(23):8629-39 PMID: 711769
Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin.
Proc Natl Acad Sci U S A. 1979 Feb;76(2):630-4 PMID: 284387
The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequence.
Cell. 1979 Apr;16(4):797-806 PMID: 455449
Specific DNA methylation sites in the vicinity of the chicken beta-globin genes.
Nature. 1979 Aug 2;280(5721):419-20 PMID: 460418
Isolation and characterization of recombinant clones containing the chicken adult beta-globin gene.
J Biol Chem. 1979 Sep 10;254(17):8099-102 PMID: 381299
Isolation of the chicken beta-globin gene and a linked embryonic beta-like globin gene from a chicken DNA recombinant library.
Cell. 1979 Aug;17(4):879-87 PMID: 158428
Conformation of ovalbumin and globin genes in chromatin during differential gene expression.
J Biol Chem. 1979 Oct 25;254(20):10532-9 PMID: 489610
The rat serum albumin gene: analysis of cloned sequences.
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3256-60 PMID: 158758

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.4.1907

Published

1980-04-00

Pages

1907-11

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC348618

Subset

External Links

PubMed source DOI: 10.1073/pnas.77.4.1907 PMC Full Text View journal details

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