Home LiteratureArticle Details
PMID: 866196 Published · ppublish English Journal Article

Renaturation kinetics of cDNA complementary to cytoplamic polyadenylated RNA from rainbow trout testis. Accessibility of transcribed genes to pancreatic DNase.

Nucleic acids research ·Vol. 4 ·No. 4 ·1977-04-00 ·Pages 883-98

Levy B, Dixon GH

Abstract

We have determined the fraction of polyadenylated cytoplasmic RNA from trout testis complementary to unique and repetitive DNA. Some 21% of the cDNA probe representative of this RNA population renatures with rapid kinetics, characteristics of repetitive sequences. The major proportion of the cDNA renatures with unique sequence DNA. Experiments with fractionated cDNA probes allow us to conclude that, in trout testis, the most abundant polyadenylated mRNAs are not preferentially transcribed from repetitive DNA, as it has shown to be the case in two eukaryotic cell lines. Treatment of trout testis nuclei with DNase I, under conditions in which 10% of the total DNA is digested, preferentially depletes the DNA of sequences being transcribed into polyadenylated mRNA. These data confirm the results of H. Weintraub and M. Groundine [(1976) Science 193, 848-856] and those of A. Garel and R. Axel [(1976) Proc. Natl. Acad. Sci. U.S.A. 73, 3966-3970] and suggest that the conformation of DNA in the active genes of chromatin is such that it is more susceptible to digestion by DNaseI.

MeSH Terms
Animals Base Sequence Chemical Phenomena Chemistry Cytoplasm DNA/metabolism Deoxyribonucleases/metabolism Deoxyribonucleotides/analysis Genes Kinetics Male Nucleic Acid Conformation Nucleic Acid Renaturation Nucleic Acids Pancreas/enzymology Poly A RNA, Messenger/biosynthesis Structure-Activity Relationship Testis Transcription, Genetic Trout
Chemicals
Deoxyribonucleotides Nucleic Acids RNA, Messenger Poly A DNA Deoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Levy B
Dixon G H
References (37)
37 references, click to expand
  1. Fidelity of chromatin transcription in vitro.
    Biochemistry. 1976 Oct 5;15(20):4356-63 PMID: 823961
  2. Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3966-70 PMID: 1069279
  3. On the existence of polyadenylated histone mRNA in Xenopus laevis oocytes.
    Cell. 1976 Oct;9(2):311-22 PMID: 987857
  4. Diversity of sequences in total and polyadenylated nuclear RNA from Drosophila cells.
    Nucleic Acids Res. 1976 Jul;3(7):1777-89 PMID: 967676
  5. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  6. Evidence for single copies of globin genes in the mouse genome.
    Nature. 1972 Sep 22;239(5369):219-21 PMID: 4117010
  7. Tissue-specific transcription of the globin gene in isolated chromatin.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3440-2 PMID: 4587253
  8. Synthesis of globin ribonucleic acid from duck-reticulocyte chromatin in vitro.
    Proc Natl Acad Sci U S A. 1973 Jul;70(7):2029-32 PMID: 4516203
  9. Cell-free transcription of mammalian chromatin: transcription of globin messenger RNA sequences from bone-marrow chromatin with mammalian RNA polymerase.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1219-23 PMID: 4364529
  10. Isolation and hybridization kinetics of messenger RNA from Dictyostelium discoideum.
    Nat New Biol. 1972 Oct 25;239(95):225-8 PMID: 4507801
  11. Organization, transcription, and regulation in the animal genome.
    Q Rev Biol. 1973 Dec;48(4):565-613 PMID: 4592421
  12. Hybridization properties of DNA sequences directing the synthesis of messenger RNA and heterogeneous nuclear RNA.
    J Cell Biol. 1971 Sep;50(3):774-86 PMID: 4999767
  13. Hybridization of ribonucleic acid with unique sequences of mouse deoxyribonucleic acid.
    Biochemistry. 1972 Feb 29;11(5):798-805 PMID: 4621823
  14. Reiteration and clustering of DNA sequences complementary to histone messenger RNA.
    Nat New Biol. 1971 Apr 7;230(14):165-9 PMID: 5279989
  15. Reiteration frequency of duck haemoglobin genes.
    Nat New Biol. 1973 Feb 14;241(111):204-7 PMID: 4512585
  16. In vitro transcription of chromatin in the presence of a mercurated nucleotide.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1564-7 PMID: 1064024
  17. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  18. Effect of estrogen on gene expression in the chick oviduct. In vitro transcription of the ovalbumin gene in chromatin.
    J Biol Chem. 1976 Jan 25;251(2):524-9 PMID: 1245487
  19. Chromatin directed transcription of 5S and tRNA genes.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1082-6 PMID: 1093165
  20. Polyadenylated RNA complementary to repetitive DNA in mouse L-cells.
    Biochemistry. 1975 Apr 8;14(7):1385-9 PMID: 1168486
  21. The metabolism of a poly(A) minus mRNA fraction in HeLa cells.
    Cell. 1974 Sep;3(1):1-10 PMID: 4213457
  22. Genes coding for polysomal 9S RNA of sea urchins: conservation and divergence.
    Nature. 1972 Nov 24;240(5378):225-8 PMID: 4569419
  23. Nonrepetitive DNA sequence representation in sea urchin embryo messenger RNA.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3516-20 PMID: 4519642
  24. Two classes of messenger RNA in cultured rat cells: repetitive sequence transcripts and unique sequence transcripts.
    J Mol Biol. 1974 Dec 25;90(4):649-63 PMID: 4475699
  25. Physical map of polyoma viral DNA fragments produced by cleavage with a restriction enzyme from Haemophilus aegyptius, endonuclease R-HaeIII.
    J Virol. 1975 Apr;15(4):946-53 PMID: 163927
  26. Co-existence of non-histone messenger RNA species lacking and containing polyadenylic acid in sea urchin embryos.
    J Mol Biol. 1974 Nov 5;89(3):435-54 PMID: 4444055
  27. The subunit structure of chromatin: characteristics of nucleohistone and nucleoprotamine from developing trout testis.
    FEBS Lett. 1974 Nov 1;48(1):156-9 PMID: 4430368
  28. Three abundance classes in HeLa cell messenger RNA.
    Nature. 1974 Jul 19;250(463):199-204 PMID: 4855195
  29. General interspersion of repetitive with non-repetitive sequence elements in the DNA of Xenopus.
    J Mol Biol. 1973 Jun 15;77(1):1-23 PMID: 4769838
  30. Synthesis of a deoxyribonucleic acid sequence complementary to ovalbumin messenger ribonucleic acid and quantification of ovalbumin genes.
    J Biol Chem. 1973 Nov 10;248(21):7530-9 PMID: 4745779
  31. The genes for silk fibroin in Bombyx mori.
    J Mol Biol. 1972 Oct 14;70(3):637-49 PMID: 5083150
  32. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.
    Science. 1968 Aug 9;161(3841):529-40 PMID: 4874239
  33. Messenger RNA complexity in Drosophila melanogaster.
    Biochemistry. 1975 Jun 3;14(11):2440-6 PMID: 806298
  34. Small RNA species of the HeLa cell: metabolism and subcellular localization.
    Cell. 1976 May;8(1):19-31 PMID: 954090
  35. Analysis of the complexity and diversity of mRNA from chicken liver and oviduct.
    Cell. 1976 Feb;7(2):247-54 PMID: 954082
  36. Structure of transcriptionally active chromatin.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4404-8 PMID: 1060119
  37. Purification and properties of biologically active rainbow trout testis protamine mRNA.
    J Biol Chem. 1976 Mar 10;251(5):1455-63 PMID: 1254578
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.4.883
Published
1977-04-00
Pages
883-98
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342492
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.4.883 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2