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PMID: 6932016 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Absence of albumin mRNA in the liver of analbuminemic rats.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 6 ·1980-06-00 ·Pages 3215-9

Esumi H, Okui M, Sato S, Sugimura T, Nagase S

Abstract

Albumin synthesis in the liver of analbuminemic rats, established as a strain from a stock of Sprague-Dawley rats, was examined in vivo by labeling protein by intraperitoneal injection of L-[3H]leucine. Albumin was not synthesized in the liver of analbuminemic rats, whereas its synthesis amounted to about 14% of the total protein synthesis in the liver of normal rats. The RNA content and size distribution of the total polysomes in the liver of analbuminemic rats were not significantly different from those of normal rats. However, no functional mRNA coding for albumin was found in poly(A)-containing RNA from the liver of analbuminemic rats when tested with a cell-free translation system derived from rabbit reticulocytes. Moreover, the amount of the RNA sequence that could hybridize to purified albumin cDNA was more than 750 times greater in the liver of normal rats than in that of analbuminemic rats.

MeSH Terms
Animals Cell-Free System Disease Models, Animal Electrophoresis, Polyacrylamide Gel Genes, Synthetic Liver/metabolism Male Protein Biosynthesis RNA, Messenger/analysis Rats Serum Albumin/biosynthesis,deficiency,genetics Transcription, Genetic
Chemicals
RNA, Messenger Serum Albumin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Esumi H
Okui M
Sato S
Sugimura T
Nagase S
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.6.3215
Published
1980-06-00
Pages
3215-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349585
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.6.3215 PMC Full Text View journal details
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