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PMID: 693321 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Chemical synthesis of a self-complementary octanucleotide, dG-G-T-T-A-A-C-C by a modified triester method.

Nucleic acids research ·Vol. 5 ·No. 8 ·1978-08-00 ·Pages 2809-23

Agarwal KL, Riftina F

Abstract

The synthesis of a self-complementary octanucleotide, d(G-G-T-T-A-A-C-C-), using a modified triester approach is described. The protected dinucleotides, d(Me2O)TribG(C1C6H4) ibG, d(Me2O)TrT(ClC6H4)T, d(Me2O)TrbzA(ClC6H4)bzA, and d(Me2O)TranC(ClC6H4)anC were synthesized by a one step triester procedure. After removal of the trityl group, the dinucleotides, dT(ClC6H4)T and danC (ClC6H4)anC were coupled to d(Me2O)TribG(ClC6H4)ibG and d(Me2O)TrbzA(ClC6H4)bzA, respectively to yield the respective tetranucleotides. The tetranucleotide, d(Me2O)TrbzA(ClC6H4)bzA(ClC6H4) and (ClC6H4)anC was detritylated and then coupled with d(Me2O)TribG(ClC6H4)ibG(ClC6H4)T(Cl6H4)T to give octanucleotide. The fully protected octanucleotide was deblocked by treatment with aqueous NH3 followed by acid and was characterized by nucleotide sequence analysis.

MeSH Terms
Base Sequence Methods Oligonucleotides/chemical synthesis Oligoribonucleotides/chemical synthesis
Chemicals
Oligonucleotides Oligoribonucleotides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Agarwal K L
Riftina F
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17 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.8.2809
Published
1978-08-00
Pages
2809-23
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342209
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.8.2809 PMC Full Text View journal details
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