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PMID: 6951190 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Assembly of nuclear ribonucleoprotein particles during in vitro transcription.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 5 ·1982-03-00 ·Pages 1469-73

Economidis IV, Pederson T

Abstract

The assembly of heterogeneous nuclear RNA (hnRNA) into ribonucleoprotein (RNP) particles has been investigated during in vitro transcription in isolated nuclei. Approximately 80% of the in vitro transcription observed in mouse Friend erythroleukemia cell nuclei is attributable to the activity of RNA polymerase II. In vitro hnRNA transcripts are assembled into particles having the same properties as the nuclear ribonucleoprotein (hnRNP) particles in which hnRNA is found in vivo. Direct contact of hnRNP proteins with newly transcribed hnRNA was demonstrated by nuclease protection experiments and by the covalent transfer of 32P-labeled nucleotides from [alpha-32P]UTP-labeled hnRNA transcripts to specific proteins by RNA--protein crosslinking followed by nuclease digestion and electrophoresis of the nucleotide-bearing proteins. The availability of an in vitro system for hnRNP assembly opens a new route for investigating the functional relationship between nuclear structure and mRNA processing.

MeSH Terms
Animals Cell Nucleus/metabolism Cell-Free System Mice Nucleoproteins/metabolism RNA Polymerase II/metabolism RNA Splicing RNA, Heterogeneous Nuclear/metabolism RNA, Messenger/metabolism Ribonucleoproteins/metabolism Transcription, Genetic
Chemicals
Nucleoproteins RNA, Heterogeneous Nuclear RNA, Messenger Ribonucleoproteins RNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Economidis I V
Pederson T
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.5.1469
Published
1982-03-00
Pages
1469-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345995
Subset
IM
Grants
NIGMS NIH HHS · GM 21595 · United States
NIGMS NIH HHS · GM 28274 · United States
PHS HHS · P30 12708 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.5.1469 PMC Full Text View journal details
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