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PMID: 6957277 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of human immunoglobulin production in vitro by prostaglandin E2.

Clinical and experimental immunology ·Vol. 49 ·No. 1 ·1982-07-00 ·Pages 115-22

Staite ND, Panayi GS

Abstract

Human peripheral blood mononuclear cells (HPBMC) from healthy individuals were stimulated polyclonally with pokeweed mitogen (PWM), in vitro, to produce IgG and IgM, which were subsequently measured by enzyme linked immunosorbent assay (ELISA). Indomethacin at 10(-6)-10(-8)M inhibited both IgG and IgM production but only when added within the first 24 hr of culture. PWM-stimulated HPBMC produced prostaglandin E2 (PGE2) during the first 24 hr of culture and this was completely inhibited by 10(-6)M indomethacin. Exogenous PGE2 added to PWM-stimulated HPBMC produced variable effects on immunoglobulin production at relatively high concentration (10(-6)-10(-7)M). However, reversal of inhibition of IgG production caused by 10(-7)M indomethacin was achieved with approximately 10(-7)M PGE2 but indomethacin-mediated inhibition of IgM production could not be fully reversed. No effect of either PGE2 or indomethacin could be detected on the PWM-induced proliferative response. These results suggest that prostaglandin E2, possibly with other arachidonic acid products, may be involved in the regulation of immunoglobulin production.

MeSH Terms

Cell Division/drug effects Cells, Cultured Dinoprostone Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin G/biosynthesis Immunoglobulin M/biosynthesis Indomethacin/pharmacology Lymphocyte Activation/drug effects Lymphocytes/immunology Pokeweed Mitogens/pharmacology Prostaglandins E/antagonists & inhibitors,pharmacology,physiology Time Factors

Chemicals

Immunoglobulin G Immunoglobulin M Pokeweed Mitogens Prostaglandins E Dinoprostone Indomethacin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Staite N D

Panayi G S

References (25)

25 references, click to expand

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Article Info

Journal

Clinical and experimental immunology

Abbr.

Clin Exp Immunol

ISSN

0009-9104

Published

1982-07-00

Pages

115-22

Language

English

Region

England

NLM ID

0057202

PMCID

PMC1536666

Subset

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PubMed source PMC Full Text View journal details

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Regulation of human immunoglobulin production in vitro by prostaglandin E2.

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