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PMID: 6957884 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 18 ·1982-09-00 ·Pages 5661-5

Woods DE, Markham AF, Ricker AT, Goldberger G, Colten HR

Abstract

cDNA clones corresponding to a major histocompatibility class III antigen, the complement protein factor B, have been isolated from a human adult liver cDNA library. The clones, ranging in size from 1.0 to 2.3 kilobases, were identified by direct hybridization with two synthetic oligonucleotide mixtures. Two regions of the factor B amino acid sequence, each with minimal ambiguity in codon assignment, were chosen for synthesis of the oligonucleotides. The sequences of two clones have been partially determined. They contain coding information for the amino acid sequence of the Bb fragment of factor B and the entire 3' -untranslated region.

MeSH Terms
Adult Amino Acid Sequence Base Sequence Cloning, Molecular Complement Factor B/genetics DNA/isolation & purification DNA, Recombinant/metabolism Enzyme Precursors/genetics Humans Liver/immunology Major Histocompatibility Complex Protein Biosynthesis RNA, Messenger/genetics,isolation & purification
Chemicals
DNA, Recombinant Enzyme Precursors RNA, Messenger DNA Complement Factor B
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Woods D E
Markham A F
Ricker A T
Goldberger G
Colten H R
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.18.5661
Published
1982-09-00
Pages
5661-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346964
Subset
IM
Grants
NIAID NIH HHS · AI15033 · United States
NHLBI NIH HHS · HL20539 · United States
NHLBI NIH HHS · HL22487 · United States
Databases
GENBANK
J00185, J00186
External Links
PubMed source DOI: 10.1073/pnas.79.18.5661 PMC Full Text View journal details
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