Indomethacin inhibits in vitro immunoglobulin production by human B-lymphocytes.
Immunoglobulin production from human B-lymphocytes stimulated in vitro with pokeweed mitogen (PWM) was measured after 11 days of cell culture. IgM and IgG levels in culture supernatants were quantified using ELISA techniques. Addition of the prostaglandin cyclooxygenase inhibitor indomethacin (10(-7) M) to cell cultures concurrently with PWM, inhibited both IgM and IgG synthesis by 80-90% in the four individuals studied. Reduction of immunoglobulin production was apparently concentration-related over the range studied (10(-6)-10(-8) M) and was not associated with cytotoxicity. If this action of indomethacin results from inhibition of cyclooxygenase, then prostaglandins or other arachidonic acid products could be involved in the regulation of human B-lymphocyte immunoglobulin production.
10 references, click to expand
-
Suppression of human T-cell mitogenesis by prostaglandin. Existence of a prostaglandin-producing suppressor cell.J Exp Med. 1977 Dec 1;146(6):1719-34 PMID: 411876
-
The effect of irradiation on T-cell suppression of ELISA-determined Ig production by human blood B-cells in vitro.Clin Exp Immunol. 1979 Nov;38(2):366-9 PMID: 316755
-
Antigen stimulation of prostaglandin synthesis and control of immune responses.Proc Natl Acad Sci U S A. 1976 Apr;73(4):1300-4 PMID: 1083526
-
Lymphocyte studies in rheumatoid arthritis. III. A comparative study of the responses of peripheral blood and synovial fluid lymphocytes to phytomitogens.Scand J Rheumatol. 1979;8(1):10-6 PMID: 432557
-
Separation of various blood cells in colloidal silica-polyvinylpyrrolidone gradients.Exp Cell Res. 1968 May;50(2):355-68 PMID: 4322973
-
Regulation of the in vitro anamnestic antibody response by cyclic AMP. IV. Evidence for participation of prostaglandins of the E series in the early events.Cell Immunol. 1979 Dec;48(2):318-28 PMID: 228869
-
Modulation of conconavalin-A-induced suppressor cell activation by prostaglandin E2.Cell Immunol. 1980 Feb;49(2):421-5 PMID: 6444371
-
Functional studies on T cells in adult human bone marrow.Clin Exp Immunol. 1979 Oct;38(1):99-105 PMID: 160850
-
Enhanced in vitro synthesis of IgM rheumatoid factor in rheumatoid arthritis.Arthritis Rheum. 1980 Sep;23(9):985-92 PMID: 6968215
-
Suppression of secondary plaque-forming cell responses by rat splenic adherent cells: evidence for dependence on prostaglandin production.Cell Immunol. 1979 Nov;48(1):195-200 PMID: 159783
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
