Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16S ribosomal RNA of Escherichia coli. IV. The effect of the methylgroups on ribosomal subunit interaction.

Nucleic acids research ·Vol. 8 ·No. 1 ·1980-01-11 ·Pages 143-51

Poldermans B, Bakker H, Van Knippenberg PH

Abstract

The effect of the presence or absence of the methylgroups of the m2(6)Am2(6)A sequence near the 3' end of 16S rRNA of Escherichia coli on the interaction of the ribosomal subunits has been studied, using wild-type (methylated) and mutant (unmethylated) ribosomes. Subunit exchange experiments and competitive association experiments show a strong preference of the 50S subunit for association with methylated 30S subunits. The results indicate that the equilibrium constant of the reaction 70S in equilibrium with 30S + 50S is dependent on the methylgroups; mutant 30S.50S couples are less stable than wild-type 30S.50S couples. It is postulated that the methylgroups also stimulate the interaction between 30S subunits and initiation factor IF-3.

MeSH Terms

Adenosine/analogs & derivatives Cloacin Escherichia coli/metabolism Kinetics Methylation Mutation RNA, Ribosomal/metabolism Ribosomes/metabolism Structure-Activity Relationship

Chemicals

RNA, Ribosomal Cloacin Adenosine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Poldermans B

Bakker H

Van Knippenberg P H

References (25)

25 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/8.1.143

Published

1980-01-11

Pages

143-51

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327248

Subset

External Links

PubMed source DOI: 10.1093/nar/8.1.143 PMC Full Text View journal details

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Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16S ribosomal RNA of Escherichia coli. IV. The effect of the methylgroups on ribosomal subunit interaction.

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