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PMID: 6988607 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fluorescence changes of a membrane-bound dye during bacteriophage T5 infection of Escherichia coli.

Journal of virology ·Vol. 33 ·No. 1 ·1980-01-00 ·Pages 553-6

Glenn J, Duckworth DH

Abstract

The fluorescence intensity of membrane-bound N-phenyl-1-naphthylamine increases dramatically when T5 bacteriophage infect colicin Ib plasmid-containing hosts. This dramatic increase is not seen during normal infections or in infections wherein either the plasmid or the phage contain mutations which allow productive infection to occur. Two smaller increases in fluorescence intensity are seen, however, in all T5 infections in which the characteristic two-step injection of DNA can proceed.

MeSH Terms
Bacteriocin Plasmids Cell Membrane/metabolism Escherichia coli/genetics,metabolism Fluorescence Fluorescent Dyes/metabolism Fluorometry T-Phages/growth & development Virus Replication
Chemicals
Fluorescent Dyes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Glenn J
Duckworth D H
References (30)
30 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.33.1.553-556.1980
Published
1980-01-00
Pages
553-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288573
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.33.1.553-556.1980 PMC Full Text View journal details
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