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PMID: 6993860 Published · ppublish English Journal Article

Characterization and cloning of plasmid like DNA of the ascomycete Podospora anserina.

Molecular & general genetics : MGG ·Vol. 178 ·No. 3 ·1980-00-00 ·Pages 639-46

Stahl U, Kück U, Tudzynski P, Esser K

Abstract

The previously reported existence of plasmid-like (pl) DNA in senescent mycelia of Podospora anserina was confirmed using new methodology. Detailed anaysis of bulk DNA has further shown a possible relationship between pl DNA and mt DNA. According to biophysical and electron microscopic experiments the pl DNA was found to consist of oligomeres having a basic unit with a contour length of 0.75 micrometer corresponding to 2.4 kb. To overcome the handicap that pl DNA is only produced in rather small amounts in the aging mycelia, this DNA was cloned in E. coli after insertion into a bacterial plasmid vector, pBR 322. It was possible to isolate a stable hybrid plasmid consisting of the vector and only one integrated monomere of pl DNA. The composition of this hybrid plasmid was confirmed by restriction endonuclease analysis and heteroduplex formation. A restriction map of the pl DNA is presented and its insertion site onto pBR 322 indicated.

MeSH Terms
Ascomycota/genetics Cloning, Molecular DNA, Bacterial/genetics DNA, Fungal/genetics DNA, Recombinant/analysis Escherichia coli/genetics Microscopy, Electron Nucleic Acid Conformation Plasmids
Chemicals
DNA, Bacterial DNA, Fungal DNA, Recombinant
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stahl U
Kück U
Tudzynski P
Esser K
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22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00337872
Published
1980-00-00
Pages
639-46
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00337872
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