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PMID: 7001368 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DNA sequence of the E. coli trpR gene and prediction of the amino acid sequence of Trp repressor.

Nucleic acids research ·Vol. 8 ·No. 7 ·1980-04-11 ·Pages 1551-60

Singleton CK, Roeder WD, Bogosian G, Somerville RL, Weith HL

Abstract

A DNA sequence of 1041 base pairs from a BamHI fragment containing the E. coli trpR gene has been determined. With this sequence and other experimental evidence, the primary structure (88 amino acids) of the Trp repressor can be predicted. Additional features of the DNA sequences include a 22 base pair region upstream from the proposed structural gene which exhibits striking homology with the trp operator, thus implying that expression of the trpR gene may be under autogenous regulation.

MeSH Terms
Amino Acid Sequence Bacterial Proteins Base Composition Base Sequence Cloning, Molecular DNA/metabolism Escherichia coli/metabolism Genes Genetic Code Protein Biosynthesis Repressor Proteins/biosynthesis Transcription Factors/biosynthesis Transcription, Genetic
Chemicals
Bacterial Proteins Repressor Proteins TRPR protein, E coli Transcription Factors DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Singleton C K
Roeder W D
Bogosian G
Somerville R L
Weith H L
References (18)
18 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.7.1551
Published
1980-04-11
Pages
1551-60
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324016
Subset
IM
Grants
NIGMS NIH HHS · 5T32GM07076 · United States
NCI NIH HHS · CA 23332 · United States
NIGMS NIH HHS · GM 22131 · United States
Databases
GENBANK
J01715, V00369
External Links
PubMed source DOI: 10.1093/nar/8.7.1551 PMC Full Text View journal details
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