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PMID: 7003308 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Amber-mutants of plasmid mini-F defective in replication.

Molecular & general genetics : MGG ·Vol. 180 ·No. 1 ·1980-00-00 ·Pages 201-4

Eichelaub R, Wehlmann H

Abstract

Amber mutants of the mini-F plasmid pML31 were isolated with the mutagen hydroxylamine. Under non-permissive conditions amber mutants segregate and show no incorporation of label into supercoiled plasmid DNA in double-label experiments. Wild-type and one mutant of mini-F were integrated by recombinant DNA techniques into the single EcoRI site of plasmid vector pBR322. Plasmid specific proteins were labeled in minicells and analysed by SDS-PAGE. A 34,000 dalton molecular weight protein was identified to be missing in the amber mutant of plasmid mini-F.

MeSH Terms
Bacterial Proteins/genetics DNA Replication DNA, Bacterial/genetics Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics F Factor Mutation Phenotype Plasmids Protein Biosynthesis Recombination, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Eichelaub R
Wehlmann H
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23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267370
Published
1980-00-00
Pages
201-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267370
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