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PMID: 7003546 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characterization of tRNA genes in tRNA region II of yeast mitochondrial DNA.

Nucleic acids research ·Vol. 8 ·No. 21 ·1980-11-11 ·Pages 5007-16

Newman D, Pham HD, Underbrink-Lyon K, Martin NC

Abstract

We have isolated individual mitochondrial tRNAs from a petite mutant OI-P2-1 known to contain a limited subset of mitochondrial tRNA genes and have mapped these genes on the wild type genome of the yeast strains MH41-7B and D273-10B. To obtain DNA for fine structure mapping and DNA sequence analysis of these genes, we screened a yeast mitochondrial DNA-pBR322 recombinant bank with the isolated tRNAs. We report here the fine structure mapping of recombinant clones containing the tryptophan, formyl methionine and proline tRNA genes as well as the DNA sequence of the proline tRNA gene. The combination of restriction mapping and DNA sequence analysis has enabled us to locate these genes precisely on the wild type genome and to determine their direction of transcription.

MeSH Terms
Base Sequence Chromosome Mapping DNA, Fungal/genetics DNA, Mitochondrial/genetics Genes RNA, Fungal/genetics RNA, Transfer/genetics Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal DNA, Mitochondrial RNA, Fungal RNA, Transfer
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Newman D
Pham H D
Underbrink-Lyon K
Martin N C
References (18)
18 references, click to expand
  1. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  3. Isoaccepting mitochondrial glutamyl-tRNA species transcribed from different regions of the mitochondrial genome of Saccharomyces cerevisiae.
    J Mol Biol. 1976 Mar 5;101(3):285-96 PMID: 768489
  4. Transfer RNA: molecular structure, sequence, and properties.
    Annu Rev Biochem. 1976;45:805-60 PMID: 60910
  5. Yeast mitochondrial DNA specifies tRNA for 19 amino acids. Deletion mapping of the tRNA genes.
    Biochemistry. 1977 Oct 18;16(21):4672-7 PMID: 334246
  6. Fine structure physical mapping of 4S RNA genes on mitochondrial DNA of Saccharomyces cerevisiae.
    Mol Gen Genet. 1977 Sep 9;154(3):255-62 PMID: 337105
  7. Study of yeast mitochondrial tRNAs by two-dimensional polyacrylamide gel electrophoresis: characterization of isoaccepting species and search for imported cytoplasmic tRNAs.
    Nucleic Acids Res. 1977 Oct;4(10):3497-510 PMID: 337238
  8. Physical mapping of genes on yeast mitochondrial DNA: localization of antibiotic resistance loci, and rRNA and tRNA genes.
    Mol Gen Genet. 1978 Jul 25;163(3):241-55 PMID: 355852
  9. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.
    Nucleic Acids Res. 1978 Aug;5(8):2721-8 PMID: 358147
  10. Physical mapping of the yeast mitochondrial genome: derivation of the fine structure and gene map of strain D273-10B and comparison with a strain (MH41-7B) differing in genome size.
    Mol Gen Genet. 1979 Feb 16;170(1):25-48 PMID: 375015
  11. The genetic map of transfer RNA genes of yeast mitochondria: correction and extension.
    Mol Gen Genet. 1979 Mar 5;170(3):261-75 PMID: 379543
  12. Sequence analysis of two yeast mitochondrial DNA fragments containing the genes for tRNA Ser UCR and tRNA Phe UUY.
    J Biol Chem. 1979 Nov 25;254(22):11735-40 PMID: 387778
  13. Assembly of the mitochondrial membrane system: sequences of yeast mitochondrial valine and an unusual threonine tRNA gene.
    Cell. 1979 Sep;18(1):47-53 PMID: 389433
  14. Compilation of tRNA sequences.
    Nucleic Acids Res. 1980 Jan 11;8(1):r1-r22 PMID: 6986608
  15. Assembly of the mitochondrial membrane system. Structure and location of the mitochondrial glutamic tRNA gene in Saccharomyces cerevisiae.
    FEBS Lett. 1980 Apr 21;113(1):52-4 PMID: 6247202
  16. Yeast mitochondrial tRNATrp can recognize the nonsense codon UGA.
    Nature. 1980 Jun 19;285(5766):579-81 PMID: 6995848
  17. Nucleotide sequence of the mitochondrial genes coding for tRNAglyGGR and tRNAvalGUR.
    Nucleic Acids Res. 1980 Mar 25;8(6):1435-42 PMID: 6253935
  18. Yeast mitochondrial methionine initiator tRNA: characterization and nucleotide sequence.
    Nucleic Acids Res. 1980 Apr 11;8(7):1445-57 PMID: 6448989
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.21.5007
Published
1980-11-11
Pages
5007-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324275
Subset
IM
Grants
NIGMS NIH HHS · GM 27597 · United States
Databases
GENBANK
J01534
External Links
PubMed source DOI: 10.1093/nar/8.21.5007 PMC Full Text View journal details
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