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PMID: 7009581 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic and biochemical characterization of periplasmic-leaky mutants of Escherichia coli K-12.

Journal of bacteriology ·Vol. 145 ·No. 3 ·1981-03-00 ·Pages 1351-8

Lazzaroni JC, Portalier RC

Abstract

Periplasmic-leaky mutants of Escherichia coli K-12 were isolated after nitrosoguanidine-induced mutagenesis. They released periplasmic enzymes into the extracellular medium. Excretion of alkaline phosphatase, which started immediately in the early exponential phase of growth, could reach up to 90% of the total enzyme production in the stationary phase. Leaky mutants were sensitive to ethylenediaminetetraacetic acid, cholic acid, and the antibiotics rifampin, chloramphenicol, mitomycin C, and ampicillin. Furthermore, they were resistant to colicin E1 and partially resistant to phage TuLa. Their genetic characterization showed that the lky mutations mapped between the suc and gal markers, near or in the tolPAB locus. A biochemical analysis of cell envelope components showed that periplasmic-leaky mutants contained reduced amounts of major outer membrane protein OmpF and increased amounts of a 16,000-dalton outer membrane protein.

MeSH Terms
Alkaline Phosphatase/metabolism Bacterial Proteins/metabolism Cell Membrane/physiology Chromosome Mapping Chromosomes, Bacterial Escherichia coli/genetics,physiology Genes Membrane Proteins/metabolism Mutation
Chemicals
Bacterial Proteins Membrane Proteins Alkaline Phosphatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lazzaroni J C
Portalier R C
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35 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.145.3.1351-1358.1981
Published
1981-03-00
Pages
1351-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217139
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.145.3.1351-1358.1981 PMC Full Text View journal details
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