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PMID: 787763 Published · ppublish English Journal Article

Heptose-deficient mutants of Escherichia coli K12 deficient in up to three major outer membrane proteins.

Molecular & general genetics : MGG ·Vol. 147 ·No. 3 ·1976-09-23 ·Pages 263-9

van Alphen W, Lugtenberg B, Berendsen W

Abstract

Mutants of Escherichia coli K12, deficient in up to three major outer membrane proteins b, c and d have been constructed. Mutants that lack the lipopolysaccharide sugar heptose are deficient in protein b. All heptose-deficient strains are supersensitive to lysozyme, various antibiotics and detergents. They excrete the periplasmic enzyme ribonuclease I. Mutants deficient in proteins c and/or d have the same sensitivity towards these compounds as the parent strain. Cells of single, double and triple mutants are all rod-shaped. Electrophoretic analysis of cell envelope proteins indicates that in some mutants the protein deficiency is partially compensated for by increased amounts of one or two of the other major outer membrane proteins. Heptose-deficient strains have an increased amount of 2-keto-3-deoxyoctonate.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacterial Proteins/metabolism Coliphages Escherichia coli/enzymology Heptoses/metabolism Muramidase/pharmacology Mutation Ribonucleases/metabolism
Chemicals
Anti-Bacterial Agents Bacterial Proteins Heptoses Ribonucleases Muramidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
van Alphen W
Lugtenberg B
Berendsen W
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35 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00582877
Published
1976-09-23
Pages
263-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00582877
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