Home LiteratureArticle Details

PMID: 7013701 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Cadmium-binding component in Escherichia coli during accommodation to low levels of this ion.

Applied and environmental microbiology ·Vol. 41 ·No. 1 ·1981-01-00 ·Pages 46-50

Khazaeli MB, Mitra RS

Abstract

An inducible cadmium-binding protein was isolated from Escherichia coli cells accommodated to 3 X 10(-6) M Cd2+ but not from normal or unaccommodated cells. Sephadex G-100, metal chelate affinity chromatography, and disc gel electrophoresis were used in the purification procedure. The molecular weight of the Cd2+-binding protein was estimated to be about 39,000 by Sephadex G-100 chromatography, making it different from the conventional, much smaller metallothionein.

MeSH Terms

Adaptation, Physiological Cadmium/isolation & purification,metabolism Carrier Proteins/isolation & purification Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Escherichia coli/analysis,metabolism Metalloproteins/isolation & purification Metallothionein/isolation & purification Molecular Weight

Chemicals

Carrier Proteins Metalloproteins cadmium-binding protein Cadmium Metallothionein

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Khazaeli M B

Mitra R S

References (14)

14 references, click to expand

Turnover of metallothioneins in rat liver.
Biochem J. 1978 Jul 15;174(1):327-38 PMID: 697759
Prokaryotic metallothionein: preliminary characterization of a blue-green alga heavy metal-binding protein.
Biochem Biophys Res Commun. 1979 Jul 12;89(1):36-43 PMID: 113007
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Estimation of the molecular weights of proteins by Sephadex gel-filtration.
Biochem J. 1964 May;91(2):222-33 PMID: 4158310
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli.
J Bacteriol. 1970 Nov;104(2):890-901 PMID: 4099097
Cadmium and zinc binding in mammalian liver and kidneys.
Arch Environ Health. 1972 Jun;24(6):419-25 PMID: 4624219
Cadmium and zinc in pregnancy and lactation.
Arch Environ Health. 1972 Jul;25(1):14-22 PMID: 5033275
Molecular mechanisms of accommodation in Escherichia coli to toxic levels of Cd2+.
J Bacteriol. 1975 Mar;121(3):1180-8 PMID: 1090597
Metal chelate affinity chromatography, a new approach to protein fractionation.
Nature. 1975 Dec 18;258(5536):598-9 PMID: 1678
Relative cadmium-binding capacity of metallothionein and other cytosolic fractions in various tissues of the rat.
Environ Physiol Biochem. 1975;5(6):378-88 PMID: 1213026
Amino-acid sequence of equine renal metallothionein-1B.
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3413-7 PMID: 1068454
Nature of the repair process associated with the recovery of Escherichia coli after exposure to Cd2+.
Biochem Biophys Res Commun. 1977 Feb 21;74(4):1450-5 PMID: 320979
Single-strand breakage in DNA of Escherichia coli exposed to Cd2+.
J Bacteriol. 1978 Jan;133(1):75-80 PMID: 338598

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.41.1.46-50.1981

Published

1981-01-00

Pages

46-50

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC243638

Subset

External Links

PubMed source DOI: 10.1128/aem.41.1.46-50.1981 PMC Full Text View journal details

Analysis Services

Cadmium-binding component in Escherichia coli during accommodation to low levels of this ion.

Contact

WeChat Official Account