Abstract
Purified, isolated yeast tRNA Ser2 was used as a hybridization probe to estimate the number of tRNA Ser2 genes in the yeast genome. Molecular clones of several of the genes were obtained. Three examples were studied in detail with respect to their genomic organization, and DNA sequences were determined for them. There appear to be eleven tRNA Ser2 genes in the yeast genome. They are neither tandemly repeated, nor clustered with other tRNA genes. They contain no intervening sequences.
MeSH Terms
Base Sequence
Cloning, Molecular
DNA, Fungal/genetics
Genes
Nucleic Acid Hybridization
RNA, Transfer, Amino Acyl/analysis,isolation & purification
Saccharomyces cerevisiae/genetics
Transcription, Genetic
Chemicals
DNA, Fungal
RNA, Transfer, Amino Acyl
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Page G S
Hall B D
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