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PMID: 7015336 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular basis of valine resistance in Escherichia coli K-12.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 2 ·1981-02-00 ·Pages 922-5

Lawther RP, Calhoun DH, Adams CW, Hauser CA, Gray J, Hatfield GW

Abstract

The relationship of valine resistance to the expression of the ilvGEDA operon of Escherichia coli K-12 has been determined. DNA sequence and in vivo protein analyses indicate that in wild-type E. coli K-12 there is a frameshift site within the gene (ilvG) for valine resistance. The ilvG+2096 (formerly designated ilv02096) mutation displaces this frameshift site, resulting in the expression of ilvG and the relief of transcriptional polarity on the distal genes of this operon. Thus, the "ilv0" mutation, which concomitantly confers valine resistance and increased expression of the ilvEDA genes, is, in fact, the "reversion" of a polar site within the first structural gene of the ilvGEDA operon.

MeSH Terms
Base Sequence DNA, Bacterial/genetics Drug Resistance, Microbial Escherichia coli/genetics,metabolism Operon Plasmids Transcription, Genetic Valine/pharmacology
Chemicals
DNA, Bacterial Valine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lawther R P
Calhoun D H
Adams C W
Hauser C A
Gray J
Hatfield G W
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.2.922
Published
1981-02-00
Pages
922-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319916
Subset
IM
Grants
NIGMS NIH HHS · GM-23182 · United States
NIGMS NIH HHS · GM-24330 · United States
NIGMS NIH HHS · GM-28021 · United States
Databases
GENBANK
J01634, J01635, M10313, M17624
External Links
PubMed source DOI: 10.1073/pnas.78.2.922 PMC Full Text View journal details
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