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PMID: 7022447 Published · ppublish English Journal Article

Expression in Escherichia coli of biologically active enzyme by a DNA sequence coding for the human plasminogen activator urokinase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 6 ·1981-06-00 ·Pages 3313-7

Ratzkin B, Lee SG, Schrenk WJ, Roychoudhury R, Chen M, Hamilton TA, Hung PP

Abstract

We have isolated clones of Escherichia coli strain K-12 that contain a hybrid pBR322 plasmid having a 4.2-kilobase insert of a DNA transcript of the mRNA of human plasminogen activator, urokinase. The bacterially produced enzyme has properties similar to those of urokinase from human fetal kidney cells. Both enzymes occur in discrete forms ranging from 32,000 to 150,000 daltons in size. They react with antibody to purified urokinase from human kidney cells, bind to a benzamidine-Sepharose column, and induce plasminogen-dependent lysis of a fibrin clot.

MeSH Terms
Base Sequence Cloning, Molecular/methods DNA, Recombinant Endopeptidases/genetics Escherichia coli/genetics Gene Expression Regulation Genes Humans Plasmids RNA, Messenger/genetics Substrate Specificity Urokinase-Type Plasminogen Activator/genetics,metabolism
Chemicals
DNA, Recombinant RNA, Messenger Endopeptidases Urokinase-Type Plasminogen Activator
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ratzkin B
Lee S G
Schrenk W J
Roychoudhury R
Chen M
Hamilton T A
Hung P P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.6.3313
Published
1981-06-00
Pages
3313-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319558
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.6.3313 PMC Full Text View journal details
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