Use of monoclonal antihapten antibodies for immunolocalisation of tissue antigens.

Journal of clinical pathology ·Vol. 34 ·No. 9 ·1981-09-00 ·Pages 1000-2

Jasani B, Thomas DW, Williams ED

Abstract

The applicability of a hapten-antihapten sandwich technique incorporating a monoclonal antihapten bridge antibody to localisation of tissue antigen is described. The results show that the method is both versatile and selective and has the potential of being even more sensitive than currently used immunoperoxidase methods.

MeSH Terms

Animals Antibodies, Monoclonal Antigens/analysis Dinitrophenols/immunology Haptens/immunology Immunoenzyme Techniques Rats

Chemicals

Antibodies, Monoclonal Antigens Dinitrophenols Haptens

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jasani B

Thomas D W

Williams E D

References (7)

7 references, click to expand

Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion.
Eur J Immunol. 1976 Jul;6(7):511-9 PMID: 825377
Hapten-sandwich labeling. III. Bifunctional reagents for immunospecific labeling of cell surface antigens.
J Immunol. 1976 Nov;117(5 Pt 1):1695-1704 PMID: 1002997
Evidence for androgen-dependent intracellular binding of prolactin in rat ventral prostate gland.
Endocrinology. 1977 Sep;101(3):929-38 PMID: 70348
Rapid preparation of peroxidase: anti-peroxidase complexes for immunocytochemical use.
J Immunol Methods. 1978;20:317-24 PMID: 418119
Immunoperoxidase technics in diagnostic pathology. Report of a workshop sponsored by the National Cancer Institute.
Am J Clin Pathol. 1979 May;71(5):483-8 PMID: 88175
Immunocytochemical technique for detection of prolactin (PRL) and growth hormone (GH) in hyperplastic and neoplastic lesions of dog prostate and mammary gland.
Histochemistry. 1979;64(3):279-86 PMID: 93102
Immunoperoxidase technique in histopathology: applications, methods, and controls.
J Clin Pathol. 1979 Oct;32(10):971-8 PMID: 93112

Article Info

Journal

Journal of clinical pathology

Abbr.

J Clin Pathol

ISSN

0021-9746

DOI

10.1136/jcp.34.9.1000

Published

1981-09-00

Pages

1000-2

Language

English

Region

England

NLM ID

0376601

PMCID

PMC494225

Subset

External Links

PubMed source DOI: 10.1136/jcp.34.9.1000 PMC Full Text View journal details

Analysis Services

Use of monoclonal antihapten antibodies for immunolocalisation of tissue antigens.

Contact

WeChat Official Account