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PMID: 702639 Published · ppublish English Journal Article

Structural studies on Rauscher murine leukemia virus: isolation and characterization of viral envelopes.

Journal of virology ·Vol. 27 ·No. 3 ·1978-09-00 ·Pages 595-603

van de Ven WJ, Vermorken AJ, Onnekink C, Bloemers HP, Bloemendal H

Abstract

A preparative method for isolating pure viral envelopes from a type-C RNA tumor virus, Rauscher murine leukemia virus, is described. Fractionation of virions of Rauscher murine leukemia virus was studied after disruption of the virions with the detergents sodium dodecyl sulfate of Nonidet P-40 in combination with ether. Fractionation was performed through flotation in a discontinuous sucrose gradient and, as appeared from electron microscopic examination, a pure viral envelope fraction was obtained in this way. By use of sensitive competition radioimmunoassays or sodium dodecyl sulfate-polyacrylamide gel electrophoresis after immunoprecipitation with polyvalent and monospecific antisera directed against Rauscher murine leukemia virus proteins, the amount of the gag and env gene-encoded structural polypeptides in the virions and the isolated envelope fraction was compared. The predominant viral structural polypeptides in the purified envelope fraction were the env gene-encoded polypeptides gp70, p15(E), and p12(E), whereas, except for p15, there was only a relatively small amount of the gag gene-encoded structural polypeptides in this fraction.

MeSH Terms
Ether/pharmacology Peptides/analysis Radioimmunoassay Rauscher Virus/drug effects,ultrastructure Surface-Active Agents/pharmacology Viral Proteins/analysis,isolation & purification
Chemicals
Peptides Surface-Active Agents Viral Proteins Ether
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
van de Ven W J
Vermorken A J
Onnekink C
Bloemers H P
Bloemendal H
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28 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.27.3.595-603.1978
Published
1978-09-00
Pages
595-603
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC525847
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.27.3.595-603.1978 PMC Full Text View journal details
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