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PMID: 7031037 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular organization of heat-labile enterotoxin genes originating in Escherichia coli of human origin and construction of heat-labile toxoid-producing strains.

Journal of bacteriology ·Vol. 148 ·No. 3 ·1981-12-00 ·Pages 983-7

Yamamoto T, Yokota T, Kaji A

Abstract

Recombinant deoxyribonucleic acid technology was employed to construct heat-labile enterotoxin (LT) toxoids. A recombinant plasmid carrying both an LT promoter region and LT subunit A (LTA) gene, lacking as much as 0.25 kilobases of the region up to the C terminus, produced a peptide possessing immunological properties of LTA but lacking the ability to construct LT activity (designated as LTA*). A cloned LT subunit B (LTB) gene produced LTB when a promoter on a vector was available for the gene. Escherichia coli producing LTA* and LTB (LT toxoids) could be useful as a vaccine.

MeSH Terms
Bacterial Toxins Cloning, Molecular DNA, Recombinant Enterotoxins/biosynthesis,genetics Escherichia coli/genetics,metabolism Escherichia coli Proteins Genes, Bacterial Plasmids Toxoids/biosynthesis,genetics
Chemicals
Bacterial Toxins DNA, Recombinant Enterotoxins Escherichia coli Proteins Toxoids heat-labile enterotoxin, E coli
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yamamoto T
Yokota T
Kaji A
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20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.148.3.983-987.1981
Published
1981-12-00
Pages
983-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216302
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.148.3.983-987.1981 PMC Full Text View journal details
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