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PMID: 7035370 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Experimental Escherichia coli ascending pyelonephritis in rats: active peroral immunization with live Escherichia coli.

Infection and immunity ·Vol. 35 ·No. 2 ·1982-02-00 ·Pages 647-53

Mattsby-Baltzer I, Hanson LA, Olling S, Kaijser B

Abstract

Peroral immunization with a live strain of Escherichia coli O6K13H1 against experimental ascending pyelonephritis caused by the same strain was studied in rats, and the effect of immunization on antibody titers against the O and K antigens and lipid A was determined. Peroral immunization with live bacteria protected significantly against pyelonephritis. Sera collected 1 week after infection from the immunized group were increased in immunoglobulin G (IgG) anti-O6 and IgM anti-K13 in comparison with the nonimmunized group. The peroral immunization did not correspondingly affect the response to lipid A. In urine, there was an IgG antibody response to the O6 antigen. In bronchopulmonary secretion, IgM, IgG, and IgA antibodies to O6 were detected. Perorally immunized animals had significantly higher levels of IgG and IgA anti-O6 compared with the nonimmunized group 1 week after infection. Passive transfer of anti-lipid A did not increase resistance against pyelonephritis.

MeSH Terms
Animals Antibodies, Bacterial/biosynthesis,immunology Antigens, Bacterial/immunology Escherichia coli/immunology Escherichia coli Infections/immunology Female Immunization Immunization, Passive Lipid A/immunology Pyelonephritis/immunology Rats
Chemicals
Antibodies, Bacterial Antigens, Bacterial Lipid A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mattsby-Baltzer I
Hanson L A
Olling S
Kaijser B
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29 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.35.2.647-653.1982
Published
1982-02-00
Pages
647-53
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC351090
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.35.2.647-653.1982 PMC Full Text View journal details
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