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PMID: 7041124 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Properties of a Saccharomyces cerevisiae mtDNA segment conferring high-frequency yeast transformation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 5 ·1982-03-00 ·Pages 1578-82

Hyman BC, Cramer JH, Rownd RH

Abstract

The bakers' yeast Saccharomyces cerevisiae is a facultative anaerobe, tolerant to mutations in its mitochondrial genome. Individual cytoplasmic petite mutants retain genetic information derived from any portion of the parenteral mtDNA, prompting questions concerning distribution of the DNA replication origin(s) on the yeast mitochondrial genome. The experiments described in this paper were designated to test the possibility of using high-frequency yeast transformation as a selection for yeast mtDNA sequences conferring autonomously replicating function. A complete petite mitochondrial genome was inserted into the yeast vector YIp5, and the hybrid plasmid (YRMp1) was used to transform yeast. YRMp1 promoted high-frequency transformation of both wild-type yeast cells and petite mutant hosts lacking mtDNA and was maintained in each of these strains as a high-copy-number extrachromosomal element. The stability and copy-number properties of YRMp1 are similar to those of YRp12, a recombinant plasmid containing a yeast chromosomal autonomously replicating sequence.

MeSH Terms
Cloning, Molecular DNA Replication DNA, Fungal/genetics DNA, Mitochondrial/genetics Extrachromosomal Inheritance Phenotype Plasmids Saccharomyces cerevisiae/genetics Transformation, Genetic
Chemicals
DNA, Fungal DNA, Mitochondrial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hyman B C
Cramer J H
Rownd R H
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.5.1578
Published
1982-03-00
Pages
1578-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346018
Subset
IM
Grants
NIGMS NIH HHS · 5 F32 GM07739 · United States
NIGMS NIH HHS · GM26527 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.5.1578 PMC Full Text View journal details
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