Home LiteratureArticle Details
PMID: 7050087 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Requirement of Fnr and NarL functions for nitrate reductase expression in Escherichia coli K-12.

Journal of bacteriology ·Vol. 151 ·No. 3 ·1982-09-00 ·Pages 1320-5

Stewart V

Abstract

I used a chlC-lac operon fusion to study regulatory mutations which affect nitrate reductase expression in Escherichia coli. A NarL- mutant apparently lacks a nitrate-specific positive regulatory component. Furthermore, an fnr (nirR) mutation prevented enzyme induction under any conditions. These data are consistent with a two-step, positive control model for nitrate reductase regulation.

MeSH Terms
DNA, Recombinant Escherichia coli/enzymology,genetics Gene Expression Regulation Genes, Bacterial Genes, Regulator Mutation Nitrate Reductases/biosynthesis,genetics Operon Oxygen/pharmacology Transduction, Genetic
Chemicals
DNA, Recombinant Nitrate Reductases Oxygen
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Stewart V
References (32)
32 references, click to expand
  1. Localization and regulation of synthesis of nitrate reductase in Escherichia coli.
    J Bacteriol. 1968 Apr;95(4):1305-13 PMID: 4869216
  2. Biochemical and genetic studies with nitrate reductase C-gene mutants of Escherichia coli.
    Mol Gen Genet. 1969;105(4):285-97 PMID: 4904136
  3. Effects of molybdate and selenite on formate and nitrate metabolism in Escherichia coli.
    J Bacteriol. 1971 Mar;105(3):1006-14 PMID: 4926673
  4. Complementation studies in the maltose-A region of the Escherichia coli K12 genetic map.
    J Mol Biol. 1971 Nov 14;61(3):681-94 PMID: 4943758
  5. Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures.
    Mol Gen Genet. 1972;116(1):1-10 PMID: 4558444
  6. Purification and properties of nitrate reductase from Escherichia coli K12.
    J Biol Chem. 1974 Aug 25;249(16):5321-7 PMID: 4137146
  7. Analysis of genetic regulatory mechanisms.
    Annu Rev Genet. 1974;8:1-13 PMID: 4613253
  8. Synthesis of nitrate reductase components in chlorate-resistant mutants of Escherichia coli.
    J Bacteriol. 1975 Mar;121(3):1117-21 PMID: 1090592
  9. The role of a novel cytochrome b-containing nitrate reductase and quinone in the in vitro reconstruction of formate-nitrate reductase activity of E. coli.
    Biochem Biophys Res Commun. 1974 Dec 23;61(4):1234-41 PMID: 4616697
  10. Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and phi80 transducing phages.
    J Mol Biol. 1975 Aug 5;96(2):307-16 PMID: 1100846
  11. Molybdenum and chlorate resistant mutants in Escherichia coli K12.
    Biochem Biophys Res Commun. 1976 Jun 7;70(3):766-73 PMID: 779782
  12. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
    J Mol Biol. 1976 Jul 5;104(3):541-55 PMID: 781293
  13. The construction in vitro of transducing derivatives of phage lambda.
    Mol Gen Genet. 1976 Jul 23;146(2):199-207 PMID: 785220
  14. Mutants of Escherichia coli K12 unable to use fumarate as an anaerobic electron acceptor.
    J Gen Microbiol. 1976 Dec;97(2):145-60 PMID: 796407
  15. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.
    J Mol Biol. 1977 Oct 15;116(1):125-59 PMID: 338917
  16. Role of the chlC gene in formation of the formate-nitrate reductase pathway in Escherichia coli.
    J Bacteriol. 1978 Feb;133(2):626-30 PMID: 342499
  17. The chromosomal location and pleiotropic effects of mutations of the nirA+ gene of Escherichia coli K12: the essential role of nirA+ in nitrite reduction and in other anaerobic redox reactions.
    J Gen Microbiol. 1978 May;106(1):1-12 PMID: 206651
  18. Laboratoire de Chimie Bactérienne C.N.R.S., Marsielle, France.
    Mol Gen Genet. 1978 Apr 6;160(2):225-9 PMID: 349355
  19. Identification of three genes controlling production of new outer membrane pore proteins in Escherichia coli K-12.
    J Bacteriol. 1978 Sep;135(3):1118-29 PMID: 357416
  20. Use of chlC-lac fusions to determine regulation of gene chlC in Escherichia coli K-12.
    J Bacteriol. 1979 Jun;138(3):726-30 PMID: 110766
  21. Fusions of the lac operon to the transfer RNA gene tyrT of Escherichia coli.
    J Mol Biol. 1979 May 25;130(3):285-301 PMID: 381675
  22. Regulation of expression of the flagellin gene (hag) in Escherichia coli K-12: analysis of hag-lac gene fusions.
    J Bacteriol. 1979 Sep;139(3):721-9 PMID: 113385
  23. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530-3 PMID: 159458
  24. The use of gene fusions to study the expression of malT the positive regulator gene of the maltose regulon.
    J Mol Biol. 1979 Aug 15;132(3):521-34 PMID: 118263
  25. Formate dehydrogenase mutants of Salmonella typhimurium: a new medium for their isolation and new mutant classes.
    Mol Gen Genet. 1979;177(1):95-101 PMID: 395418
  26. Isolation and characterization of lambda specialized transducing bacteriophages carrying Klebsiella pneumoniae nif genes.
    J Bacteriol. 1980 Mar;141(3):1264-71 PMID: 6245064
  27. Linkage map of Escherichia coli K-12, edition 6.
    Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
  28. Nitrate reductase and cytochrome bnitrate reductase structural genes as parts of the nitrate reductase operon.
    Mol Gen Genet. 1981;181(4):535-40 PMID: 7022129
  29. Operon fusions in the nitrate reductase operon and study of the control gene nir R in Escherichia coli.
    Mol Gen Genet. 1981;182(3):477-9 PMID: 6457962
  30. Molybdenum cofactor requirement for biotin sulfoxide reduction in Escherichia coli.
    J Bacteriol. 1982 Feb;149(2):469-78 PMID: 6460021
  31. Fusions of flagellar operons to lactose genes on a mu lac bacteriophage.
    J Bacteriol. 1982 Apr;150(1):16-26 PMID: 7037746
  32. Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci.
    J Bacteriol. 1982 Aug;151(2):788-99 PMID: 7047497
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.151.3.1320-1325.1982
Published
1982-09-00
Pages
1320-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC220410
Subset
IM
Grants
NIGMS NIH HHS · GM-18006 · United States
NIGMS NIH HHS · GM7082 · United States
External Links
PubMed source DOI: 10.1128/jb.151.3.1320-1325.1982 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2