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PMID: 7051003 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mutational specificity of UV light in Escherichia coli: indications for a role of DNA secondary structure.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 13 ·1982-07-00 ·Pages 4123-7

Todd PA, Glickman BW

Abstract

We used the lacI forward mutagenesis system to determine the mutational specificity of UV-induced mutation in a repair-proficient (Uvr+) and a repair-deficient (delta UvrB) strain of Escherichia coli. The spectra recovered at similar levels of mutagenesis were similar, the exception being a mutational hotspot at site A24 specific to the delta UvrB strain. Mutations induced at this hotspot, as well as those induced at other mutational hotspots that were found to be common to both the Uvr+ and Uvr- strains, involve G . C leads to A . T transitions. All of the hotspots are at sites of potential dipyrimidine photoproducts, such as thymine-cytosine and cytosine-cytosine dimers, or of pyrimidine-cytosine photoproduct Py-C lesions. Each of these hotspots occurs at a site in the potential hairpin loop of quasi-palindromic sequences. These observations suggest an important role for DNA structure in determining the fate of UV-induced premutational lesions.

MeSH Terms
Base Sequence DNA, Bacterial/radiation effects Escherichia coli/radiation effects Mutation Nucleic Acid Conformation Pyrimidine Dimers/radiation effects Ultraviolet Rays
Chemicals
DNA, Bacterial Pyrimidine Dimers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Todd P A
Glickman B W
References (33)
33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.13.4123
Published
1982-07-00
Pages
4123-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346589
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.13.4123 PMC Full Text View journal details
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