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PMID: 7051005 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Repair and mutagenesis of plasmid DNA modified by ultraviolet irradiation or N-acetoxy-N-2-acetylaminofluorene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 13 ·1982-07-00 ·Pages 4133-7

Schmid SE, Daune MP, Fuchs RP

Abstract

Plasmid DNA was modified in vitro to various extents with N-acetoxy-N-2-acetylaminofluorene or UV irradiation. The modified plasmid DNAs were then used to transform Escherichia coli strains having different repair capabilities. Both survival and mutagenesis frequencies of the plasmid were measured as a function of the number of lesions per plasmid molecule. The majority of N-2-acetylaminofluorene (AAF) adducts, like thymine dimers, were repaired by the excision (uvrA+-dependent) pathway. In rec+ strains, dose-dependent mutagenesis occurred in either AAF- or UV-modified plasmid DNA. This is in contrast with results obtained in recA- strains, in which only AAF adducts gave rise to a lower, but dose-dependent, mutagenesis frequency. In these recA- strains there was no UV mutagenesis. Unlike what is observed with phages, induction of the "SOS" functions by UV irradiation of the bacteria prior to transformation did not increase the survival or the mutagenesis of the plasmid.

MeSH Terms
2-Acetylaminofluorene/analogs & derivatives Acetoxyacetylaminofluorene/pharmacology DNA Repair Escherichia coli/drug effects,genetics,radiation effects Kinetics Mutation Plasmids/drug effects,radiation effects Transformation, Bacterial Ultraviolet Rays
Chemicals
Acetoxyacetylaminofluorene 2-Acetylaminofluorene
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schmid S E
Daune M P
Fuchs R P
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.13.4133
Published
1982-07-00
Pages
4133-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346591
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.13.4133 PMC Full Text View journal details
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