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PMID: 7096448 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Polymorphism of actin paracrystals induced by polylysine.

The Journal of cell biology ·Vol. 93 ·No. 2 ·1982-05-00 ·Pages 452-8

Fowler WE, Aebi U

Abstract

We describe a method for the induction of different polymorphic forms of actin filament paracrystals. This polymorphism is probably based on differences in the stagger and/or polarity of adjacent filaments in single-layered paracrystals and by superposition of different layers in multilayered paracrystals. The helical parameters defining the filament geometry are indistinguishable for the different polymorphic forms observed and for the four different actins used. Analysis of these paracrystals, some of which are ordered to better than 2.5 nm, should provide a reference structure suitable for alignment and orientation within the actin filament of high resolution models of the actin monomer obtained from crystal data.

MeSH Terms
Actins Crystallization Macromolecular Substances Microscopy, Electron Polylysine Potassium Chloride
Chemicals
Actins Macromolecular Substances Polylysine Potassium Chloride
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fowler W E
Aebi U
References (24)
24 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.93.2.452
Published
1982-05-00
Pages
452-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112856
Subset
IM
Grants
NIGMS NIH HHS · GM-27765 · United States
External Links
PubMed source DOI: 10.1083/jcb.93.2.452 PMC Full Text View journal details
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