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PMID: 7097853 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Viral gene products in adenovirus type-2 transformed hamster cells.

Journal of virology ·Vol. 41 ·No. 3 ·1982-03-00 ·Pages 1076-82

Esche H

Abstract

I have analyzed viral gene products expressed in five adenovirus type 2 (Ad2)- cytoplasmic, viral RNA which was selected by hybridization to cloned restriction endonuclease fragments of Ad2 DNA. Proteins synthesized in vitro were analyzed by electrophoresis in sodium dodecyl sulfate-polyacrylamide gels and compared with those directed by RNAs prepared from productively infected cells. The early regions E1 and E4 of adenovirus type 2 (Ad2) were found to be expressed in all of five Ad2-transformed hamster embryo cells lines. RNA transcribed from early region E2, which codes for the 72,000-molecular-weight (72K) DNA-binding protein was detected in cell line HE1 only, and early region E3 was expressed exclusively in cell line HE4. RNA transcribed from the region between approximately 12 and 35 map units, coding for immediate early (13.5K, 52/53K) and immediate early proteins (13.6K, 16K, 17K, 87K), as well as RNA from late genes, was not found in any of the cell lines HE1 to HE5 had electrophoretic mobilities similar to those programmed by RNA from productively infected cells.

MeSH Terms

Adenoviruses, Human/genetics Animals Cell Line Cell Transformation, Viral Cricetinae DNA, Viral/analysis Embryo, Mammalian Genes, Viral Protein Biosynthesis RNA, Messenger/analysis RNA, Viral/analysis Transcription, Genetic Viral Proteins/analysis

Chemicals

DNA, Viral RNA, Messenger RNA, Viral Viral Proteins

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Esche H

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23 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.41.3.1076-1082.1982

Published

1982-03-00

Pages

1076-82

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC256846

Subset

External Links

PubMed source DOI: 10.1128/JVI.41.3.1076-1082.1982 PMC Full Text View journal details

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