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PMID: 711327 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Specific binding of staphylococcal alpha-toxin to isolated rabbit vagus nerves in vitro.

Infection and immunity ·Vol. 21 ·No. 3 ·1978-09-00 ·Pages 1024-6

Szmigielski S, Harshman S

Abstract

The binding of staphylococcal [125I]alpha-toxin to rabbit vagus nerves in vitro was a saturable process. The radiolabeled alpha-toxin binding was reduced by the coaddition of added navive alpha-toxin, indicating that the binding is specific. Sucrose gradient analysis of detergent-extracted complexes of [125I]alpha-toxin-rabbit vagus nerves showed both high and low S-value peaks analogous to those observed with similarly treated alpha-toxin-rabbit erythrocyte preparations (P. Cassidy and S. Harshman, Biochemistry, in press).

MeSH Terms
Animals Bacterial Toxins/metabolism Binding Sites In Vitro Techniques Kinetics Rabbits Staphylococcus Vagus Nerve/metabolism
Chemicals
Bacterial Toxins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Szmigielski S
Harshman S
References (7)
7 references, click to expand
  1. Enhancement of hemolytic and cytotoxic activity of staphylococcal alpha-toxin in vitro by incubation with cultured fibroblasts. Brief communication.
    Zentralbl Bakteriol Orig A. 1978 Apr;240(3):297-301 PMID: 664979
  2. The binding of tetrodotoxin to nerve membranes.
    J Physiol. 1971 Feb;213(1):235-54 PMID: 5575342
  3. Neurotoxicity of staphylococcal alpha- and beta-hemolysins.
    Contrib Microbiol Immunol. 1973;1:328-44 PMID: 4616789
  4. The binding of staphylococcal 125I-alpha-toxin (B) to erythrocytes.
    J Biol Chem. 1973 Aug 10;248(15):5545-6 PMID: 4806341
  5. Purification of staphylococcal alpha-toxin by adsorption chromatography on glass.
    Infect Immun. 1976 Mar;13(3):982-6 PMID: 1270140
  6. Studies on the binding of staphylococcal 125I-labeled alpha-toxin to rabbit erythrocytes.
    Biochemistry. 1976 Jun 1;15(11):2348-55 PMID: 1276142
  7. Use of lactoperoxidase catalyzed iodination in immunochemical studies.
    Immunochemistry. 1971 Mar;8(3):289-97 PMID: 5170158
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.21.3.1024-1026.1978
Published
1978-09-00
Pages
1024-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC422099
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.21.3.1024-1026.1978 PMC Full Text View journal details
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