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PMID: 7127901 Published · ppublish English Journal Article

Auto-oxidative damage in Behçet's disease--endothelial cell damage following the elevated oxygen radicals generated by stimulated neutrophils.

Clinical and experimental immunology ·Vol. 49 ·No. 1 ·1982-07-00 ·Pages 247-55

Niwa Y, Miyake S, Sakane T, Shingu M, Yokoyama M

Abstract

The functions of phagocytes are enhanced in patients with Behçet's disease, therefore, we investigated the neutrophil-derived oxygen intermediates (OI) and lysosomal enzymes from 17 patients receiving glucocorticosteroids (steroids) and colchicine. Cultured endothelial cells were incubated with neutrophils to assess tissue injury. In cases of the complete type, in the active stage of the disease, OI production was markedly increased. The other patients showed significantly higher OI and higher lysosomal enzyme levels than patients with other diseases (controls) receiving drug therapy. Cytotoxicity tests showed that the 51Cr release was also significantly higher. The destruction of desmosomes and cell deformation were demonstrated electron microscopically. The simultaneous addition of superoxide dismutase and catalase in the cell culture decreased the 51Cr release to control levels. These findings suggest that neutrophils from patients with Behçet's disease generate high levels of OI, resulting in endothelial tissue damage.

MeSH Terms
Adolescent Adult Behcet Syndrome/blood,immunology,pathology Cytotoxicity, Immunologic Endothelium/ultrastructure Female Glucuronidase/biosynthesis Humans Hydrogen Peroxide/blood Luminescent Measurements Male Microscopy, Electron Middle Aged Muramidase/biosynthesis Neutrophils/immunology,metabolism Oxygen Consumption
Chemicals
Hydrogen Peroxide Muramidase Glucuronidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Niwa Y
Miyake S
Sakane T
Shingu M
Yokoyama M
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37 references, click to expand
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Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
Published
1982-07-00
Pages
247-55
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1536650
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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