Structural characteristics of tetanolysin and its binding to lipid vesicles.

Journal of bacteriology ·Vol. 152 ·No. 2 ·1982-11-00 ·Pages 888-92

Rottem S, Cole RM, Habig WH, Barile MF, Hardegree MC

Abstract

Tetanolysin binding to lipid vesicles was found to depend on the molar ratio of cholesterol to phospholipid, being low in vesicles containing up to 20 mol% cholesterol and high in vesicles containing more than 33 mol%. High concentrations of purified tetanolysin preparations formed arc- and ring-shaped structures. The structures were not readily detectable in diluted preparations unless incubated with lipid vesicles containing high molar ratios of cholesterol to phospholipid. It is suggested that the toxin is concentrated on the vesicles to local concentrations high enough to form the arcs and rings.

MeSH Terms

Bacterial Toxins Cholesterol Dimyristoylphosphatidylcholine Kinetics Liposomes Microscopy, Electron Phosphatidylcholines Tetanus Toxin

Chemicals

Bacterial Toxins Liposomes Phosphatidylcholines Tetanus Toxin tetanolysin Cholesterol Dimyristoylphosphatidylcholine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Rottem S

Cole R M

Habig W H

Barile M F

Hardegree M C

References (17)

17 references, click to expand

Symmetrical distribution and rapid transbilayer movement of cholesterol in Mycoplasma gallisepticum membranes.
Biochim Biophys Acta. 1981 Dec 21;649(3):572-80 PMID: 7317418
SEPARATION OF NEUROTOXIN AND HEMOLYSIN OF CLOSTRIDIUM TETANI.
Proc Soc Exp Biol Med. 1965 Jun;119:405-8 PMID: 14328904
Tetanolysin: in-vivo effects in animals.
J Infect Dis. 1971 Jan;123(1):51-60 PMID: 4925417
Lateral phase separations in binary mixtures of cholesterol and phospholipids.
Biochem Biophys Res Commun. 1973 Jul 17;53(2):446-51 PMID: 4352068
Effect of tetanolysin on platelets and lysosomes.
Infect Immun. 1974 Apr;9(4):696-701 PMID: 4595758
Effect of streptolysin O on erythrocyte membranes, liposomes, and lipid dispersions. A protein-cholesterol interaction.
J Cell Biol. 1975 Oct;67(1):160-74 PMID: 1176529
Interaction between tetanolysin and Mycoplasma cell membrane.
Biochim Biophys Acta. 1976 Dec 14;455(3):876-88 PMID: 793633
Deuterium nuclear magnetic resonance investigation of the dipalmitoyl lecithin-cholesterol-water system.
J Am Chem Soc. 1977 Oct 26;99(22):7353-5 PMID: 578806
Alteration by cereolysin of the structure of cholesterol-containing membranes.
Biochim Biophys Acta. 1978 Feb 21;507(2):230-41 PMID: 414780
Equilibrium studies of lecithin-cholesterol interactions I. Stoichiometry of lecithin-cholesterol complexes in bulk systems.
Biophys J. 1978 Jun;22(3):469-88 PMID: 667296
Role of cholesterol in the action of cereolysin on membranes.
Arch Biochem Biophys. 1978 Oct;190(2):603-10 PMID: 102256
Lateral diffusion in binary mixtures of cholesterol and phosphatidylcholines.
Proc Natl Acad Sci U S A. 1979 Jan;76(1):15-8 PMID: 284326
Cholesterol-dependent tetanolysin damage to liposomes.
Biochim Biophys Acta. 1979 Feb 20;551(1):224-8 PMID: 427152
Ring formation of perfringolysin O as revealed by negative stain electron microscopy.
Biochim Biophys Acta. 1979 Dec 12;558(3):307-13 PMID: 228722
Effect of streptolysin O and digitonin on egg lecithin/cholesterol vesicles.
Biochim Biophys Acta. 1980 Jul 16;600(1):91-102 PMID: 6249363
Purification and some properties of tetanolysin.
Microbiol Immunol. 1980;24(7):575-84 PMID: 6997696
Dilatometry of dipalmitoyllecithin-cholesterol bilayers.
Biochemistry. 1980 Oct 14;19(21):4828-34 PMID: 6893555

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.152.2.888-892.1982

Published

1982-11-00

Pages

888-92

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC221544

Subset

External Links

PubMed source DOI: 10.1128/jb.152.2.888-892.1982 PMC Full Text View journal details

Analysis Services

Structural characteristics of tetanolysin and its binding to lipid vesicles.

Contact

WeChat Official Account