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PMID: 7152675 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stimulation of migration of human monocytes by bacterial cell walls and muramyl peptides.

Infection and immunity ·Vol. 38 ·No. 3 ·1982-12-00 ·Pages 817-24

Ogawa T, Kotani S, Fukuda K, Tsukamoto Y, Mori M, Kusumoto S, Shiba T

Abstract

Bacterial cell walls, water-soluble fragments of the wall peptidoglycan, N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP), and 6-O-acyl derivatives of MDP were examined for migration-stimulating activity on human peripheral blood monocytes by using a multiwell chemotaxis assembly. Cell walls isolated from 11 bacterial species caused a definite increase in monocyte migration, but the walls of Micrococcus lysodeikticus were scarely active. The migration-enhancing activity of Staphylococcus epidermidis cell walls was retained by a monomer as well as a polymer of disaccharide peptides which were prepared by digestion of the peptidoglycan with enzymes. It was finally revealed that the migration of monocytes was enhanced by MDP. 6-O-Octadecanoyl-MDP, 6-O-(2-tetradecylhexadecanoyl)-MDP, and 6-O-(3-hydroxy-2-docosylhexacosanoyl)-N-acetylmuramyl-L-seryl-D-isoglutamine were active, but to a lesser extent. A checkerboard assay demonstrated that the increased monocyte migration caused by S. epidermidis cell walls was directed toward a positive stimulus (chemotaxis).

MeSH Terms
Acetylmuramyl-Alanyl-Isoglutamine/pharmacology Cell Wall/physiology Chemotaxis, Leukocyte/drug effects Lactobacillus/ultrastructure Monocytes/physiology Mycobacterium/ultrastructure Nocardia/ultrastructure Peptidoglycan/pharmacology Staphylococcus/ultrastructure Streptococcus/ultrastructure
Chemicals
Peptidoglycan Acetylmuramyl-Alanyl-Isoglutamine
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ogawa T
Kotani S
Fukuda K
Tsukamoto Y
Mori M
Kusumoto S
Shiba T
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39 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.38.3.817-824.1982
Published
1982-12-00
Pages
817-24
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC347821
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.38.3.817-824.1982 PMC Full Text View journal details
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