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PMID: 7177853 Published · ppublish English Journal Article

New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.

Nucleic acids research ·Vol. 10 ·No. 21 ·1982-11-11 ·Pages 6675-94

Efimov VA, Reverdatto SV, Chakhmakhcheva OG

Abstract

A rapid and convenient method for the synthesis of deoxyribooligonucleotides has been developed using the phosphotriester approach. The advantage of this methodology for work in solution was successfully demonstrated in synthesis of a number of DNA fragments up to 32-long. Adaptation of the presented method to solid-phase synthesis allows a pentadecamer to be assembled in 4-5 hours using dinucleotides as coupling units.

MeSH Terms
Base Sequence Chromatography, High Pressure Liquid DNA/chemical synthesis Indicators and Reagents Kinetics Methods Oligodeoxyribonucleotides/chemical synthesis Oligonucleotides/chemical synthesis Organophosphates Organophosphorus Compounds Solutions
Chemicals
Indicators and Reagents Oligodeoxyribonucleotides Oligonucleotides Organophosphates Organophosphorus Compounds Solutions DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Efimov V A
Reverdatto S V
Chakhmakhcheva O G
References (18)
18 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.21.6675
Published
1982-11-11
Pages
6675-94
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326957
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.21.6675 PMC Full Text View journal details
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